catalog number :
MBS700975
products type :
ELISA Kit
products full name :
Pig Interleukin 12, IL-12/P40 ELISA Kit
products short name :
interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
products name syn :
Pig Interleukin 12; IL-12/P40 ELISA KIT; CLMF; CLMF2; IL-12B; NKSF; NKSF2; IL12; subunit p40; interleukin 12; p40; interleukin 12B; interleukin-12 beta chain; natural killer cell stimulatory factor; 40 kD subunit; interleukin 12B (natural killer cell stimulatory factor 2; cytotoxic lymphocyte maturation factor 2; p40)
other names :
interleukin-12 subunit beta; Interleukin-12 subunit beta; interleukin-12 subunit beta; IL-12B; CLMF p40; IL-12 subunit p40; interleukin-12 p40 subunit; cytotoxic lymphocyte maturation factor 40 kDa subunit; Cytotoxic lymphocyte maturation factor 40 kDa subunit; CLMF p40; IL-12 subunit p40
products gene name :
IL12B
other gene names :
IL12B; IL12B; IL-12B; CLMF p40
uniprot entry name :
IL12B_PIG
specificity :
This assay has high sensitivity and excellent specificity for detection of pig IL-12/P40. No significant cross-reactivity or interference between pig IL-12/P40 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: The minimum detectable dose of pig IL-12/P40 is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-12/P40 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-12/P40 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-12/P40 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-12/P40 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_999178.1
ncbi gb acc num :
NM_214013.1
ncbi mol weight :
36,829 Da
ncbi pathways :
African Trypanosomiasis Pathway (194379); African Trypanosomiasis Pathway (194323); Allograft Rejection Pathway (84530); Allograft Rejection Pathway (535); Amoebiasis Pathway (167313); Amoebiasis Pathway (167191); Chagas Disease (American Trypanosomiasis) Pathway (147807); Chagas Disease (American Trypanosomiasis) Pathway (147795); Cytokine-cytokine Receptor Interaction Pathway (84463); Cytokine-cytokine Receptor Interaction Pathway (460)
uniprot summary :
Function: Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC
size4 :
10x96-Strip-Wells