ELISA/assay

Human Proteolysis Inducing Factor/Dermcidin, PIF/DCD ELISA Kit

MBS700974

48-Strip-Wells

565 USD

ELISA Kit

Human Proteolysis Inducing Factor/Dermcidin, PIF/DCD ELISA Kit

dermcidin

Human Proteolysis Inducing Factor/Dermcidin (PIF/DCD) ELISA Kit; AIDD; DCD-1; DSEP; HCAP; MGC71930; PIF; diffusible survival/evasion peptide; preproteolysin; proteolysis inducing factor; survival promoting peptide; dermcidin

dermcidin isoform 1 preproprotein; Dermcidin; dermcidin; preproteolysin; survival promoting peptide; proteolysis inducing factor; diffusible survival/evasion peptide; dermcidin; PreproteolysinCleaved into the following 2 chains:Survival-promoting peptide; DCD-1

DCD

DCD; DCD; PIF; AIDD; DSEP; HCAP; DCD-1; AIDD; DSEP

DCD_HUMAN

Human

110

This assay has high sensitivity and excellent specificity for detection of human PIF/DCD. No significant cross-reactivity or interference between human PIF/DCD and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates, urine, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.625 ng/ml -40 ng/ml. Sensitivity: 0.156 ng/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PIF/DCD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PIF/DCD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIF/DCD is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIF/DCD bound in the initial step. The color development is stopped and the intensity of the color is measured.

16751921

NP_444513.1

NM_053283.3

P81605

11,284 Da

Metabolic Pathways (132022); Pyrimidine Deoxyribonuleotide Biosynthesis, CDP/CTP = DCDP/dCTP,dTDP/dTTP Pathway (430005); Pyrimidine Deoxyribonuleotide Biosynthesis, CDP/CTP = DCDP/dCTP,dTDP/dTTP Pathway (468277); Pyrimidine Metabolism Pathway (4550); Pyrimidine Metabolism Pathway (309)

This gene encodes a secreted protein that is subsequently processed into mature peptides of distinct biological activities. The C-terminal peptide is constitutively expressed in sweat and has antibacterial and antifungal activities. The N-terminal peptide, also known as diffusible survival evasion peptide, promotes neural cell survival under conditions of severe oxidative stress. A glycosylated form of the N-terminal peptide may be associated with cachexia (muscle wasting) in cancer patients. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014]

Function: DCD-1 displays antimicrobial activity thereby limiting skin infection by potential pathogens in the first few hours after bacterial colonization. Highly effective against E.coli, E.faecalis, S.aureus and C.albicans. Optimal pH and salt concentration resemble the conditions in sweat. Also exhibits proteolytic activity.1 PublicationManual assertion based on experiment in:Ref.5. Catalytic activity: Preferential cleavage: Arg- -Xaa, Lys- -Xaa. Cofactor: Manganese. Required for survival-promoting peptide.

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150