catalog number :
MBS700955
products type :
ELISA Kit
products full name :
Human Heat Shock Protein 70, HSP-70 ELISA Kit
products short name :
[heat shock 70kDa protein 1B]
products name syn :
[Human Heat Shock Protein 70; HSP-70 ELISA Kit; DAAP-21F2.7; FLJ54328; HSP70-1B; HSP70-2; HSPA1A; heat shock 70kD protein 1B; heat shock 70kDa protein 1B]
other names :
[heat shock 70 kDa protein 1B; Heat shock 70 kDa protein 1B; heat shock 70 kDa protein 1B; HSP70.2; chaperone; heat shock 70 kD protein 2; 70 kda heat shock protein-2; heat shock 70 kDa protein 2; heat shock 70kDa protein 1B; heat-shock 70-kilodalton protein 1B; Heat shock 70 kDa protein 2; HSP70.2]
products gene name :
[HSPA1B]
other gene names :
[HSPA1A; HSPA1B; HSPA2; HSPA1B; HSP70-2; HSP70-2; HSP70.2]
uniprot entry name :
HS71B_BOVIN
specificity :
This assay has high sensitivity and excellent specificity for detection of human Hpt. No significant cross-reactivity or interference between human Hpt and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Urine. Assay Type: Quantitative Sandwich. Detection Range: 1.563 ng/ml-100 ng/ml. Sensitivity: 1.044 ng/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Hpt has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Hpt present is bound by the immobilized antibody. After removing any unbound substances, a Horseradish Peroxidase (HRP)-conjugated antibody specific for Hpt is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Hpt bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_976067.2
ncbi gb acc num :
NM_203322.2
ncbi mol weight :
70,228 Da
ncbi pathways :
AUF1 (hnRNP D0) Destabilizes MRNA Pathway (1009263); Androgen Receptor Signaling Pathway (198553); Antigen Processing And Presentation Pathway (84313); Antigen Processing And Presentation Pathway (485); Apoptosis Modulation By HSP70 Pathway (198554); Attenuation Phase Pathway (1009515); Cellular Response To Heat Stress Pathway (1009512); Cellular Responses To Stress Pathway (942950); Endocytosis Pathway (102272); Endocytosis Pathway (102181)
uniprot summary :
Function: In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage
size1 :
24-Strip-Wells (LIMIT 1)
size5 :
10x96-Strip-Wells