Human cysteine-rich 61, Cyr61/CCN1 ELISA Kit | MyBioSource MBS700945 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human cysteine-rich 61, Cyr61/CCN1 ELISA Kit

catalog :

MBS700945

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS700945

products type :

ELISA Kit

products full name :

Human cysteine-rich 61, Cyr61/CCN1 ELISA Kit

products short name :

cysteine-rich, angiogenic inducer, 61

products name syn :

Human cysteine-rich 61 (Cyr61/CCN1) ELISA Kit; CCN1; GIG1; IGFBP10; cysteine-rich heparin-binding protein 61; cysteine-rich; anigogenic inducer; 61; cysteine-rich; angiogenic inducer; 61

other names :

protein CYR61; Protein CYR61; protein CYR61; IBP-10; IGFBP-10; CCN family member 1; IGF-binding protein 10; cysteine-rich, anigogenic inducer, 61; cysteine-rich heparin-binding protein 61; insulin-like growth factor-binding protein 10; cysteine-rich, angiogenic inducer, 61; CCN family member 1; Cysteine-rich angiogenic inducer 61; Insulin-like growth factor-binding protein 10; IBP-10; IGF-binding protein 10; IGFBP-10; Protein GIG1

products gene name :

CYR61

other gene names :

CYR61; CYR61; CCN1; GIG1; IGFBP10; CCN1; GIG1; IGFBP10; IBP-10; IGF-binding protein 10; IGFBP-10

uniprot entry name :

CYR61_HUMAN

reactivity :

Human

sequence length :

381

specificity :

This assay has high sensitivity and excellent specificity for detection of human Cyr61. No significant cross-reactivity or interference between human Cyr61 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, saliva, tissue homogenates. Assay Type: Sandwich. Detection Range: 78 pg/ml -5000 pg/ml. Sensitivity: The minimum detectable dose of human Cyr61 is typically less than 19.5 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Cyr61 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Cyr61 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Cyr61 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Cyr61 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

31542331

ncbi acc num :

NP_001545.2

ncbi gb acc num :

NM_001554.4

uniprot acc num :

O00622

ncbi mol weight :

42,027 Da

ncbi pathways :

Hypertrophy Model Pathway (198828); Oncostatin M Signaling Pathway (711361); Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway (169352); RhoA Signaling Pathway (169356); Amb2 Integrin Signaling Pathway (137945)

ncbi summary :

The secreted protein encoded by this gene is growth factor-inducible and promotes the adhesion of endothelial cells. The encoded protein interacts with several integrins and with heparan sulfate proteoglycan. This protein also plays a role in cell proliferation, differentiation, angiogenesis, apoptosis, and extracellular matrix formation. [provided by RefSeq, Sep 2011]

uniprot summary :

Function: Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3.1 PublicationManual assertion based on experiment in:Ref.10

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage