product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Mouse gamma-Secretase ELISA Kit
catalog :
MBS700934
quantity :
48-Strip-Wells
price :
565 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS700934
products type :
ELISA Kit
products full name :
Mouse gamma-Secretase ELISA Kit
products short name :
anterior pharynx defective 1 homolog B (C. elegans)
products name syn :
Mouse gamma-Secretase ELISA kit; APH-1B; DKFZp564D0372; FLJ33115; PRO1328; PSFL; TAAV688; anterior pharynx defective 1B-like; presenilin stabilization factor-like; anterior pharynx defective 1 homolog B (C. elegans)
other names :
gamma-secretase subunit APH-1B; Gamma-secretase subunit APH-1B; gamma-secretase subunit APH-1B; APH-1b; aph-1beta; APH1B homolog; anterior pharynx defective 1c homolog; anterior pharynx defective 1b homolog (C. elegans); Aph-1beta
products gene name :
APH1B
other gene names :
Aph1b; Aph1b; Aph1c; 2310057K14Rik; APH-1b
uniprot entry name :
APH1B_MOUSE
reactivity :
Mouse
sequence length :
257
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse gamma -Secretase. No significant cross-reactivity or interference between mouse gamma -Secretase and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma and tissue homogenates. Assay Type: Sandwich. Detection Range: 7.8 pg/ml -500 pg/ml. Sensitivity: The minimum detectable dose of mouse gamma -Secretase is typically less than 1.95 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for gamma-Secretase has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any gamma-Secretase present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for gamma-Secretase is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of gamma-Secretase bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
29243936
ncbi acc num :
NP_808251.1
ncbi gb acc num :
NM_177583.4
uniprot acc num :
Q8C7N7
ncbi mol weight :
28,703 Da
ncbi pathways :
Activated NOTCH1 Transmits Signal To The Nucleus Pathway (1000681); Alzheimer's Disease Pathway (83294); Alzheimer's Disease Pathway (509); Axon Guidance Pathway (970585); Cell Death Signalling Via NRAGE, NRIF And NADE Pathway (1000892); Constitutive Signaling By NOTCH1 HD+PEST Domain Mutants Pathway (1000688); Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway (1000686); Delta-Notch Signaling Pathway (198316); Developmental Biology Pathway (970813); Disease Pathway (970606)
uniprot summary :
Function: Probable subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex. Probably present in a minority of gamma-secretase complexes compared to APH1A
size1 :
48-Strip-Wells
price1 :
565 USD
size2 :
96-Strip-Wells
price2 :
810
size3 :
5x96-Strip-Wells
price3 :
2750
size4 :
10x96-Strip-Wells
price4 :
5150
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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