ELISA/assay

rat follicle-stimulating hormone, FSH ELISA Kit

MBS700927

96 Strip Wells

690 USD

ELISA Kit

rat follicle-stimulating hormone, FSH ELISA Kit

follicle-stimulating hormone, FSH

rat follicle-stimulating hormone; FSH ELISA Kit; follicle-stimulating hormone; FSH

FSH; Bromodomain-containing protein 2; O27.1.1; Really interesting new gene 3 protein

FSH

BRD2; KIAA9001; RING3

BRD2_HUMAN

Rat

801

This assay has high sensitivity and excellent specificity for detection of rat FSH. No significant cross-reactivity or interference between rat FSH and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates. Detection Range: 0.25 mlU/ml-100 mlU/ml. Detection Wavelength: 450 nm. Sensitivity: 0.25 mlU/ml

Sample Volume: 50-100ul

Introduction: Follicle-stimulating hormone (FSH) is a hormone synthesized and secreted by gonadotropes in the anterior pituitary gland. FSH regulates the development, growth, pubertal maturation, and reproductive processes of the human body. FSH and Luteinizing hormone (LH) act synergistically in reproduction. FSH is a glycoprotein. Each monomeric unit is a protein molecule with a sugar attached to it; two of these make the full, functional protein. Its structure is similar to those of LH, TSH, and hCG. The protein dimer contains 2 polypeptide units, labeled alpha and beta subunits. The alpha subunits of LH, FSH, TSH, and hCG are identical, and contain 92 amino acids. The beta subunits vary. FSH has a beta subunit of 118 amino acids (FSHB), which confers its specific biologic action and is responsible for interaction with the FSH-receptor. The sugar part of the hormone is composed of fucose, galactose, mannose, galactosamine, glucosamine, and sialic acid, the latter being critical for its biologic half-life. The half-life of FSH is 3-4 hours. Its molecular wt is 30000. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with a goat-anti-rabbit antibody. Standards or samples are then added to the appropriate microtiter plate wells with a HRP-conjugated FSH and antibody preparation specific for FSH and incubated. Then substrate solutions are added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of FSH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

2980663

CAA43996.1

P25440

74,881 Da

Focal Adhesion Pathway 83067!!Focal Adhesion Pathway 478!!MAPK Signaling Pathway 83048!!MAPK Signaling Pathway 456!!Salmonella Infection Pathway 375172!!Salmonella Infection Pathway 375149

Function: May play a role in spermatogenesis or folliculogenesis Binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling. Regulates transcription of the CCND1 gene. Plays a role in nucleosome assembly.1 PublicationManual assertion based on experiment in:Ref.8

96 Strip Wells

690 USD