Human Adipose Triglyceride Lipase, ATGL ELISA Kit | MyBioSource MBS700918 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Adipose Triglyceride Lipase, ATGL ELISA Kit

catalog :

MBS700918

quantity :

96 Strip Wells

price :

850 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS700918

products type :

ELISA Kit

products full name :

Human Adipose Triglyceride Lipase, ATGL ELISA Kit

products short name :

patatin-like phospholipase domain containing 2

products name syn :

Human Adipose Triglyceride Lipase; ATGL ELISA kit; 1110001C14Rik; ATGL; DESNUTRIN; DKFZp667M109; FP17548; PEDF-R; TTS-2.2; TTS2; adipose triglyceride lipase; pigment epithelium-derived factor; transport-secretion protein 2.2; triglyceride hydrolase; patatin-like phospholipase domain containing 2

other names :

patatin-like phospholipase domain-containing protein 2; Patatin-like phospholipase domain-containing protein 2; patatin-like phospholipase domain-containing protein 2; TTS2.2; desnutrin; IPLA2-zeta; triglyceride hydrolase; adipose triglyceride lipase; transport-secretion protein 2.2; pigment epithelium-derived factor; calcium-independent phospholipase A2; patatin-like phospholipase domain containing protein 2; patatin-like phospholipase domain containing 2; Adipose triglyceride lipase; Calcium-independent phospholipase A2; Desnutrin; IPLA2-zeta; Pigment epithelium-derived factor; TTS2.2; Transport-secretion protein 2; TTS2

products gene name :

PNPLA2

other gene names :

PNPLA2; PNPLA2; ATGL; TTS2; PEDF-R; FP17548; TTS-2.2; iPLA2zeta; 1110001C14Rik; ATGL; FP17548; TTS2

uniprot entry name :

PLPL2_HUMAN

reactivity :

Human

sequence length :

504

specificity :

This assay has high sensitivity and excellent specificity for detection of human ATGL. No significant cross-reactivity or interference between human ATGL and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: For the quantitative determination of human adipose triglyceride lipase (ATGL) concentrations in serum, urine, tissue homogenates. Detection Range: 6.25 mIU/ml -400 mIU/ml.

other info2 :

Sensitivity: The minimum detectable dose of human ATGL is typically less than 1.56 mIU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ATGL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ATGL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ATGL is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ATGL bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

32698724

ncbi acc num :

NP_065109.1

ncbi gb acc num :

NM_020376.3

uniprot acc num :

Q96AD5

ncbi mol weight :

55,316 Da

ncbi pathways :

Acyl Chain Remodeling Of DAG And TAG Pathway 645315!!Acylglycerol Degradation Pathway 413388!!Acylglycerol Degradation Pathway 468272!!Fatty Acid Beta Oxidation Pathway 198865!!Glycerolipid Metabolism Pathway 82986!!Glycerolipid Metabolism Pathway 361!!Glycerophospholipid Biosynthesis Pathway 645313!!Metabolic Pathways 132956!!Metabolism Pathway 477135!!Metabolism Of Lipids And Lipoproteins Pathway 160976

ncbi summary :

This gene encodes an enzyme which catalyzes the first step in the hydrolysis of triglycerides in adipose tissue. Mutations in this gene are associated with neutral lipid storage disease with myopathy. [provided by RefSeq, Jul 2010]

uniprot summary :

Function: Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. Also has acylglycerol transacylase activity. May act coordinately with LIPE/HLS within the lipolytic cascade. Regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion.3 PublicationsManual assertion based on experiment in:Ref.1. Catalytic activity: Triacylglycerol + H2O = diacylglycerol + a carboxylate. Enzyme regulation: Inhibited by BEL ((E)-6-(bromomethylene)-3-(1-naphthalenyl)-2H-tetrahydropyran-2-one), a suicide substrate inhibitor. No differences in enzymatic activity that uses (1,2-dilinoleoyl)-phosphatidylcholine as substrate was detected in the presence or absence of ATP. Activated by ABHD5 and SERPINF1.2 PublicationsManual assertion based on experiment in:Ref.6

size :

96 Strip Wells

price :

850 USD

more info or order :

MyBioSource product webpage