ELISA/assay

Pig Proinsulin, PI ELISA Kit

MBS700914

48-Strip-Wells

590 USD

ELISA Kit

Pig Proinsulin, PI ELISA Kit

Proinsulin, PI

Pig Proinsulin; PI ELISA Kit; Proinsulin; PI

proinsulin; Insulin; insulin; proinsulin; preproinsulin; insulin-dependent diabetes mellitus 2; insulin

PI

INS; INS; ILPR; IRDN; IDDM1; IDDM2; MODY10

INS_HUMAN

Pig

110

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PI. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for PI and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain PI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of PI in the samples is then determined by comparing the O.D. of the samples to the standard curve.

59036749

AAW83741.1

P01308

21,537 Da

AGE/RAGE Pathway (698754); AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); ATF-2 Transcription Factor Network Pathway (138006); Adipogenesis Pathway (198832); Aldosterone-regulated Sodium Reabsorption Pathway (130626); Aldosterone-regulated Sodium Reabsorption Pathway (130590); Amyloids Pathway (366238); Arf6 Trafficking Events Pathway (137954); Cardiac Progenitor Differentiation Pathway (712094)

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]

Function: Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

48-Strip-Wells

590 USD

96-Strip-Wells

850

5x96-Strip-Wells

2880

10x96-Strip-Wells

5440