ELISA/assay

Rat fatty acid binding protein 4, adipocyte, FABP4 ELISA Kit

MBS700911

48-Strip-Wells

565 USD

ELISA Kit

Rat fatty acid binding protein 4, adipocyte, FABP4 ELISA Kit

fatty acid binding protein 4, adipocyte

Rat fatty acid binding protein 4; adipocyte (FABP4) ELISA kit; A-FABP; aP2; ; fatty acid binding protein 4; adipocyte

Fatty acid-binding protein, adipocyte; Fatty acid-binding protein, adipocyte; fatty acid-binding protein, adipocyte; AFABP; A-FABP; fatty acid-binding protein 4; adipocyte lipid-binding protein; adipocyte-type fatty acid-binding protein; fatty acid binding protein 4, adipocyte; Adipocyte lipid-binding protein; ALBP; Adipocyte-type fatty acid-binding protein; A-FABP; AFABP; Fatty acid-binding protein 4

FABP4

Fabp4; Fabp4; aP2; Albp; ALBP; A-FABP; AFABP

FABP4_RAT

Rat

132

This assay has high sensitivity and excellent specificity for detection of rat FABP4. No significant cross-reactivity or interference between rat FABP4 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates and Cell lysates Cell lysates Cell lysates Cell lysates Cell lysates Cell lysates Cell lysates. Assay Type: Sandwich. Detection Range: 1.56 ng/ml -100 ng/ml. Sensitivity: The minimum detectable dose of rat FABP4 is typically less than 0.39 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FABP4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FABP4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FABP4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FABP4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

2494405

P70623.3

P70623

14,708 Da

Developmental Biology Pathway (1009870); Hormone-sensitive Lipase (HSL)-mediated Triacylglycerol Hydrolysis Pathway (1010690); Lipid Digestion, Mobilization, And Transport Pathway (1010683); Metabolism Pathway (1010675); Metabolism Of Lipids And Lipoproteins Pathway (1010682); PPAR Signaling Pathway (83434); PPAR Signaling Pathway (450); Transcriptional Regulation Of White Adipocyte Differentiation Pathway (1009920)

intracellular lipid-binding protein; mediates fatty acid trafficking during lipolysis [RGD, Feb 2006]

Function: Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150