ELISA/assay

Rat bone alkaline phosphatase, BALP ELISA Kit

MBS700904

48-Strip-Wells

565 USD

ELISA Kit

Rat bone alkaline phosphatase, BALP ELISA Kit

bone alkaline phosphatase, BALP

Rat bone alkaline phosphatase; BALP ELISA Kit; bone alkaline phosphatase; BALP

BALP

Rat

This assay has high sensitivity and excellent specificity for detection of rat BALP. No significant cross-reactivity or interference between rat BALP and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 U/L -1000 U/L. Sensitivity: 3.9 U/L

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BALP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BALP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BALP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BALP bound in the initial step. The color development is stopped and the intensity of the color is measured.

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150