Human thymidinephosphorylase, TP ELISA Kit | MyBioSource MBS700893 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human thymidinephosphorylase, TP ELISA Kit

catalog :

MBS700893

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS700893

products type :

ELISA Kit

products full name :

Human thymidinephosphorylase, TP ELISA Kit

products short name :

thymidine phosphorylase

products name syn :

Human thymidinephosphorylase; TP ELISA Kit; ECGF1; MNGIE; PDECGF; TP; hPD-ECGF; OTTHUMP00000196769; endothelial cell growth factor 1 (platelet-derived) ; gliostatin; thymidine phosphorylase

other names :

thymidine phosphorylase isoform 1 proprotein; Thymidine phosphorylase; thymidine phosphorylase; tdRPase; gliostatin; thymidine phosphorylase; Gliostatin; Platelet-derived endothelial cell growth factor; PD-ECGF; TdRPase

products gene name :

TYMP

other gene names :

TYMP; TYMP; TP; ECGF; ECGF1; MNGIE; MEDPS1; MTDPS1; PDECGF; hPD-ECGF; ECGF1; TP; PD-ECGF

uniprot entry name :

TYPH_HUMAN

reactivity :

Human

sequence length :

482

specificity :

This assay has high sensitivity and excellent specificity for detection of human TP. No significant cross-reactivity or interference between human TP and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 62.5 ng/ml -4000 ng/ml. Sensitivity: The minimum detectable dose of human TP is typically less than 15.6 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TP bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

166158922

ncbi acc num :

NP_001107227.1

ncbi gb acc num :

NM_001113755.2

uniprot acc num :

P19971

ncbi mol weight :

49,955 Da

ncbi pathways :

Bladder Cancer Pathway (83115); Bladder Cancer Pathway (527); Drug Metabolism - Other Enzymes Pathway (83033); Drug Metabolism - Other Enzymes Pathway (428); Fluoropyrimidine Activity Pathway (198778); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Nucleotides Pathway (106263); Pyrimidine Catabolism Pathway (106288); Pyrimidine Metabolism Pathway (106281)

ncbi summary :

This gene encodes an angiogenic factor which promotes angiogenesis in vivo and stimulates the in vitro growth of a variety of endothelial cells. It has a highly restricted target cell specificity acting only on endothelial cells. Mutations in this gene have been associated with mitochondrial neurogastrointestinal encephalomyopathy. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Apr 2012]

uniprot summary :

Function: May have a role in maintaining the integrity of the blood vessels. Has growth promoting activity on endothelial cells, angiogenic activity in vivo and chemotactic activity on endothelial cells in vitro.1 PublicationManual assertion based on experiment in:Ref.8. Catalytic activity: Thymidine + phosphate = thymine + 2-deoxy-alpha-D-ribose 1-phosphate.

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage