ELISA/assay

Mouse inhibin B, INH-B ELISA Kit

MBS700883

96 Strip Wells

895 USD

ELISA Kit

Mouse inhibin B, INH-B ELISA Kit

inhibin B

Mouse inhibin B; INH-B ELISA Kit; EDF; FRP; FSH-releasing protein; Inhibin; beta-1; OTTHUMP00000207260; erythroid differentiation factor; follicle-stimulating hormone-releasing protein; inhibin beta A; inhibin; beta A (activin A; activin; inhibin B

inhibin beta A chain; Inhibin beta A chain; inhibin beta A chain; activin beta-A chain; inhibin beta-A; Activin beta-A chain

INH-B

Inhba; Inhba

INHBA_MOUSE

Mouse

424

This assay has high sensitivity and excellent specificity for detection of Mouse INH-B. No significant cross-reactivity or interference between Mouse INH-B and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Range: 0.8 pg/ml-200 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 0.2 pg/ml

Sample Volume: 50-100ul

Introduction: Inhibin is a peptide that is an inhibitor of FSH synthesis and secretion, and participates in the regulation of the menstrual cycle. Inhibin contains an alpha and beta subunit linked by disulfidebonds. Two forms of inhibin differ in their beta subunits (A or B), while their alpha subunits are identical. Inhibin belongs to the transforming growth factor-beta (TGF-beta) superfamily. In women, FSH stimulates the secretion of inhibin from the granulosa cells of the ovarian follicles in ovary. In turn, inhibin suppresses FSH.Inhibin secretion is diminished by GnRH, and enhanced by insulin-like growth factor-1 (IGF-1). Inhibins have been defined based on their activity of suppressing pituitary gonadotropin secretion. Thus, the serum concentrations of Inhibin B and FSH are inversely correlated, and at low serum levels of Inhibin B, FSH concentration goes up. Inhibin B serum concentration increases gradually in the follicular phase to a broad peak at 7 days prior to the LH surge, and may constitute the limiting factor for the duration of the inter-cycle FSH rise. A decrease and a second peak follow this peak one-day past the LH surge. Most of the serum Inhibin B concentration originates from large follicles, since such follicles secrete 10 fold higher concentrations than do the small follicles. Inhibin B concentrations drop to a very low concentration at the luteal phase, and is undetectable during pregnancy. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated INH-B and antibody preparation specific for INH-B, and incubated. Then substrate solutions are added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of INH-B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

6680451

NP_032406.1

NM_008380.1

Q04998

47,392 Da

Antagonism Of Activin By Follistatin Pathway 1000997!!Cytokine-cytokine Receptor Interaction Pathway 83248!!Cytokine-cytokine Receptor Interaction Pathway 460!!Glycoprotein Hormones Pathway 1000421!!Metabolism Of Proteins Pathway 970583!!Peptide Hormone Biosynthesis Pathway 1000420!!Peptide Hormone Metabolism Pathway 1000413!!Signal Transduction Pathway 970540!!Signaling By Activin Pathway 1000996!!TGF Beta Signaling Pathway 198393

Function: Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.

96 Strip Wells

895 USD