Rat Insulin-like growth factor binding protein 1, IGFBP-1 ELISA Kit | MyBioSource MBS700876 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Insulin-like growth factor binding protein 1, IGFBP-1 ELISA Kit

catalog :

MBS700876

quantity :

48-Strip-Wells

price :

465 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS700876

products type :

ELISA Kit

products full name :

Rat Insulin-like growth factor binding protein 1, IGFBP-1 ELISA Kit

products short name :

insulin-like growth factor binding protein 1

products name syn :

Rat Insulin-like growth factor binding protein 1; IGFBP-1 ELISA Kit; AFBP; IBP1; IGF-BP25; PP12; hIGFBP-1; IGF-binding protein 1; alpha-pregnancy-associated endometrial globulin; amniotic fluid binding protein; binding protein-25; binding protein-26; binding protein-28; gr; insulin-like growth factor binding protein 1

other names :

insulin-like growth factor-binding protein 1; Insulin-like growth factor-binding protein 1; insulin-like growth factor-binding protein 1; IBP-1; IGFBP-1; placental protein 12; IGF-binding protein 1; insulin-like growth factor binding protein 1

products gene name :

IGFBP1

other gene names :

Igfbp1; Igfbp1; IBP1; IGFBA; IGF-BP25; Igfbp-1; IBP-1; IGF-binding protein 1; IGFBP-1

uniprot entry name :

IBP1_RAT

reactivity :

Rat

sequence length :

272

specificity :

This assay has high sensitivity and excellent specificity for detection of Rat IGFBP1. No significant cross-reactivity or interference between Rat IGFBP1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates and tissue homogenates. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: 3.9 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

products description :

Introduction: Insulin-like growth factor-binding protein 1 is a protein that in humans is encoded by the IGFBP1 gene. This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein bindsboth insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IGFBP-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for IGFBP-1 and Avidin conjugated to HorseradishPeroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain IGFBP-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IGFBP-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

6981080

ncbi acc num :

NP_037276.1

ncbi gb acc num :

NM_013144.1

uniprot acc num :

P21743

ncbi mol weight :

29,684 Da

ncbi pathways :

ATF4 Activates Genes Pathway (1009788); Metabolism Of Proteins Pathway (1009706); Myometrial Relaxation And Contraction Pathways (198465); PERK Regulates Gene Expression Pathway (1009787); Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway (1009781); Unfolded Protein Response (UPR) Pathway (1009782)

ncbi summary :

a modulator of a Insulin growth factor (IGF) bioavailability [RGD, Feb 2006]

uniprot summary :

Function: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration

size1 :

48-Strip-Wells

price1 :

465 USD

size2 :

96-Strip-Wells

price2 :

655

size3 :

5x96-Strip-Wells

price3 :

2455

size4 :

10x96-Strip-Wells

price4 :

4595

more info or order :

MyBioSource product webpage