ELISA/assay

Rat gonadotropin-releasing hormone, GnRH ELISA Kit

MBS700875

48-Strip-Wells

605 USD

ELISA Kit

Rat gonadotropin-releasing hormone, GnRH ELISA Kit

gonadotropin-releasing hormone 1 (luteinizing-releasing hormone)

Rat gonadotropin-releasing hormone; GnRH ELISA Kit; GNRH; GRH; LHRH; LNRH; gonadotropin-releasing hormone 1; gonadotropin-releasing hormone 1 (leutinizing-releasing hormone) ; gonadotropin-releasing hormone 1 (lutienizing hormone-releasing hormone) ; gonadotropin-releasing hormone 1 (luteinizing-releasing hormone)

progonadoliberin-1; Progonadoliberin-1; progonadoliberin-1; progonadoliberin I; gonadotropin releasing hormone 1; luteinizing hormone-releasing hormone; gonadotropin-releasing hormone 1 (luteinizing-releasing hormone); Progonadoliberin ICleaved into the following 2 chains:Gonadoliberin-1Alternative name(s):Gonadoliberin I; Gonadotropin-releasing hormone I; GnRH-I; Luliberin I; Luteinizing hormone-releasing hormone I; LH-RH I

GNRH1

Gnrh1; Gnrh1; Gnrh; Lhrh; Gnrha; Rgnrhg1; Gnrh; GnRH-I; LH-RH I

GON1_RAT

Rat

92

This assay has high sensitivity and excellent specificity for detection of rat GnRH. No significant cross-reactivity or interference between rat GnRH and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Competitive. Detection Range: 2 pg/ml -800 pg/ml. Sensitivity: The minimum detectable dose of rat GnRH is typically less than 2pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest rat GnRH concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for GnRH and Horseradish Peroxidase (HRP) conjugated GnRH. The competitive inhibition reaction is launched between with HRP labeled GnRH and unlabeled GnRH with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of GnRH in the sample. The color development is stopped and the intensity of the color is measured.

6980968

NP_036899.1

NM_012767.2

P07490

10,500 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (1010164); G Alpha (q) Signalling Events Pathway (1010197); GPCR Downstream Signaling Pathway (1010193); GPCR Ligand Binding Pathway (1010163); Gastrin-CREB Signalling Pathway Via PKC And MAPK (1010213); GnRH Signaling Pathway (83483); GnRH Signaling Pathway (502); Hormone Ligand-binding Receptors Pathway (1010178); Signal Transduction Pathway (1010015); Signaling By GPCR Pathway (1010162)

involved in induction of ovulation; may inhibit anterior pituitary cell apoptosis [RGD, Feb 2006]

Function: Stimulates the secretion of gonadotropins; it stimulates the secretion of both luteinizing and follicle-stimulating hormones.

48-Strip-Wells

605 USD

96-Strip-Wells

870

5x96-Strip-Wells

2950

10x96-Strip-Wells

5535