Human Mast Cell Carboxypeptidase ELISA Kit | MyBioSource MBS700873 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Mast Cell Carboxypeptidase ELISA Kit

catalog :

MBS700873

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS700873

products type :

ELISA Kit

products full name :

Human Mast Cell Carboxypeptidase ELISA Kit

products short name :

carboxypeptidase A3 (mast cell)

products name syn :

Human Mast Cell Carboxypeptidase ELISA Kit; MC-CPA; carboxypeptidase A3; carboxypeptidase A3 (mast cell)

other names :

mast cell carboxypeptidase A; Mast cell carboxypeptidase A; mast cell carboxypeptidase A; tissue carboxypeptidase A; carboxypeptidase A3 (mast cell); Carboxypeptidase A3

products gene name :

CPA3

other gene names :

CPA3; CPA3; MC-CPA; MC-CPA

uniprot entry name :

CBPA3_HUMAN

reactivity :

Human

sequence length :

417

specificity :

This assay has high sensitivity and excellent specificity for detection of human mast cell carboxypeptidase. No significant cross-reactivity or interference between human mast cell carboxypeptidase and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml -20 ng/ml. Sensitivity: The minimum detectable dose of human mast cell carboxypeptidase is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for mast cell carboxypeptidase has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any mast cell carboxypeptidase present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for mast cell carboxypeptidase is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of mast cell carboxypeptidase bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

221316749

ncbi acc num :

NP_001861.2

ncbi gb acc num :

NM_001870.2

uniprot acc num :

P15088

ncbi mol weight :

48,670 Da

ncbi pathways :

Metabolism Of Angiotensinogen To Angiotensins Pathway (685555); Metabolism Of Proteins Pathway (106230); Pancreatic Secretion Pathway (169306); Pancreatic Secretion Pathway (169295); Peptide Hormone Metabolism Pathway (771603); Protein Digestion And Absorption Pathway (172847); Protein Digestion And Absorption Pathway (171868); Renin-angiotensin System Pathway (83075); Renin-angiotensin System Pathway (486)

ncbi summary :

Three different forms of human pancreatic procarboxypeptidase A have been isolated. This gene encodes a form which is obtained as a binary complex of a procarboxypeptidase A with proproteinase E and functions as a secretory granule metalloexopeptidase. [provided by RefSeq, Jan 2009]

uniprot summary :

CPA3: Three different forms of human pancreatic procarboxypeptidase A have been isolated. This gene encodes a form which is obtained as a binary complex of a procarboxypeptidase A with proproteinase E and functions as a secretory granule metalloexopeptidase. [provided by RefSeq, Jan 2009]. Protein type: EC 3.4.17.1; Protease. Chromosomal Location of Human Ortholog: 3q24. Cellular Component: transport vesicle; extracellular region; secretory granule. Molecular Function: zinc ion binding; metallocarboxypeptidase activity. Biological Process: cellular protein metabolic process; proteolysis; angiotensin maturation

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage