catalog number :
MBS700871
products type :
ELISA Kit
products full name :
Rat heat shock protein, HSP47 ELISA Kit
products short name :
serpin peptidase inhibitor, clade H (heat shock protein 47) , member 1, (collagen binding protein 1)
products name syn :
Rat heat shock protein; HSP47 ELISA Kit; AsTP3; CBP1; CBP2; HSP47; PIG14; PPROM; RA-A47; SERPINH2; gp46; arsenic-transactivated protein 3; colligin-1; colligin-2; proliferation-inducing gene 14; rheumatoid arthritis antigen A-47; serine (or cys; serpin peptidase inhibitor; clade H (heat shock protein 47); member 1; (collagen binding protein 1)
other names :
serpin H1; Serpin H1; serpin H1; GP46; colligin; collagen-binding protein; 47 kDa heat shock protein; collagen binding protein 2; serpin peptidase inhibitor, clade H, member 1; serine (or cysteine) peptidase inhibitor, clade H, member 1; serine (or cysteine) proteinase inhibitor, clade H, member 1; serine proteinase inhibitor, clade H (heat shock protein 47), member 1; serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1; serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 2; serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1); 47 kDa heat shock protein; Collagen-binding protein; Colligin; GP46
products gene name :
SERPINH1
other gene names :
Serpinh1; Serpinh1; Cbp2; Serpinh2; Cbp1; Hsp47; Colligin
uniprot entry name :
SERPH_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of rat HSP47. No significant cross-reactivity or interference between rat HSP47 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: 0.039 ng/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HSP47 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HSP47 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HSP47 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HSP47 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_058869.1
ncbi gb acc num :
NM_017173.1
ncbi mol weight :
46,518 Da
ncbi pathways :
Collagen Biosynthesis And Modifying Enzymes Pathway (1009925); Collagen Formation Pathway (1009924); Endochondral Ossification Pathway (219771); Extracellular Matrix Organization Pathway (1009923)
ncbi summary :
binds collagen; may act as a molecular chaperone [RGD, Feb 2006]
uniprot summary :
Function: Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
size4 :
10x96-Strip-Wells