catalog number :
MBS700870
products type :
ELISA Kit
products full name :
Rat glial fibrillary acidic protein, GFAP ELISA Kit
products short name :
glial fibrillary acidic protein
products name syn :
Rat glial fibrillary acidic protein; GFAP ELISA Kit; FLJ45472; ; glial fibrillary acidic protein
other names :
glial fibrillary acidic protein; Glial fibrillary acidic protein; glial fibrillary acidic protein; intermediate filament; glial fibrillary acidic protein alpha; glial fibrillary acidic protein
products gene name :
GFAP
other gene names :
Gfap; Gfap; GFAP
uniprot entry name :
GFAP_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of rat GFAP. No significant cross-reactivity or interference between rat GFAP and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 78pg/ml-5000 pg/ml. Sensitivity: Typically less than 19.5 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GFAP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GFAP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GFAP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GFAP bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_058705.2
ncbi gb acc num :
NM_017009.2
ncbi mol weight :
49,957 Da
ncbi pathways :
Nuclear Signaling By ERBB4 Pathway (1010137); Signal Transduction Pathway (1010015); Signaling By ERBB4 Pathway (1010134); Spinal Cord Injury Pathway (739006)
ncbi summary :
intermediate filament protein specific for astrocytes [RGD, Feb 2006]
uniprot summary :
Function: GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
size4 :
10x96-Strip-Wells