ELISA/assay

Human D-Lactate Dehydrogenase, D-LDH ELISA Kit

MBS700863

96 Strip Wells

760 USD

ELISA Kit

Human D-Lactate Dehydrogenase, D-LDH ELISA Kit

lactate dehydrogenase D

Human D-Lactate Dehydrogenase; D-LDH ELISA Kit; MGC57726; D-lactate dehydrogenase; lactate dehydrogenase D

probable D-lactate dehydrogenase, mitochondrial isoform 1; Probable D-lactate dehydrogenase, mitochondrial; probable D-lactate dehydrogenase, mitochondrial; lactate dehydrogenase D

LDHD

LDHD; LDHD; DLD; DLD; Lactate dehydrogenase D

LDHD_HUMAN

Human

507

This assay has high sensitivity and excellent specificity for detection of Human LDHD. No significant cross-reactivity or interference between Human LDHD and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates. Detection Range: 31.25 mIU/ml-2000 mIU/ml. Detection Wavelength: 450 nm. Sensitivity: 7.8 mIU/ml

Sample Volume: 50-100ul

Introduction: Lactate dehydrogenase (LDH) is an enzyme present in a wide variety of organisms, including plants and animals. LDH is found in the liver, kidneys, striated muscle, and heart muscle that catalyze the reversible conversion of pyruvate and lactate. It catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. It converts pyruvate, the final product of glycolysis to lactic acid when oxygen is absent or in short supply, and it performs the reverse reaction during the cori cycle in the liver. At high concentrations of lactate, the enzyme exhibits feedback inhibition and the rate of conversion of pyruvate to lactate is decreased. Tissue breakdown elevates levels of LDH, and therefore a measure of it indicates e.g. hemolysis. Other disorders indicated by elevated LDH include cancer, meningitis, encephalitis, acute pancreatitis and HIV. Two forms of lactate dehydrogenase with different substrate specificities have been found; L-lactate dehydrogenase and D-lactate dehydrogenase. Whereas the enzyme from vertebrates and plants only forms L-lactate, invertebrates, lower fungi, and prokaryotic organisms possess either a D- or L-lactate specific dehydrogenase, depending on the species. Some prokaryotes even possess both enzymes within the same cell. On the other hand, eukaryotes have several isozyme forms, but all have a single stereospecificity. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to D-LDH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for D-LDH and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well 4 and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain D-LDH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of D-LDH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

37595754

NP_705690.2

NM_153486.3

Q86WU2

54,871 Da

Metabolism Of Proteins Pathway 106230!!Mitochondrial Protein Import Pathway 576261!!Pyruvate Metabolism Pathway 82998!!Pyruvate Metabolism Pathway 373!!Methylglyoxal Degradation I Pathway 142313!!Methylglyoxal Degradation VI Pathway 547501!!Methylglyoxal Degradation VI Pathway 139067

The protein encoded by this gene belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. The similar protein in yeast has both D-lactate and D-glycerate dehydrogenase activities. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]

96 Strip Wells

760 USD