catalog number :
MBS700849
products type :
ELISA Kit
products full name :
Chicken Interleukin 18, IL-18 ELISA Kit
products short name :
interleukin 18 (interferon-gamma-inducing factor)
products name syn :
Chicken Interleukin 18; IL-18 ELISA KIT; IGIF; IL-18; IL-1g; IL1F4; MGC12320; IL-1 gamma; interferon-gamma-inducing factor; interleukin 18; interleukin-1 gamma; interleukin-18; interleukin 18 (interferon-gamma-inducing factor)
other names :
Interleukin-18; Interleukin-18; interleukin-18; interleukin 18 (interferon-gamma-inducing factor)
products gene name :
IL18
other gene names :
IL18; IL18; IL-18; ChIL-18; IL-18
uniprot entry name :
IL18_CHICK
specificity :
This assay has high sensitivity and excellent specificity for detection of chicken IL-18. No significant cross-reactivity or interference between chicken IL-18 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 7.8 pg/ml -500 pg/ml. Sensitivity: The minimum detectable dose of chicken IL-18 is typically less than 1.95 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-18 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-18 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-18 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-18 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi mol weight :
22,787 Da
ncbi pathways :
Cytokine-cytokine Receptor Interaction Pathway (119307); Cytokine-cytokine Receptor Interaction Pathway (460); Cytosolic DNA-sensing Pathway (125152); Cytosolic DNA-sensing Pathway (124833); Influenza A Pathway (217175); Influenza A Pathway (217150); NOD-like Receptor Signaling Pathway (122198); NOD-like Receptor Signaling Pathway (122131); Salmonella Infection Pathway (375175); Salmonella Infection Pathway (375149)
uniprot summary :
Function: Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells
size4 :
10x96-Strip-Wells