ELISA/assay

Human Alpha-fetoprotein, AFP ELISA Kit

MBS700843

96 Strip Wells

655 USD

ELISA Kit

Human Alpha-fetoprotein, AFP ELISA Kit

alpha-fetoprotein

Human Alpha-fetoprotein; AFP ELISA Kit; FETA; HPAFP; OTTHUMP00000160480; alpha-1-fetoprotein; alpha-fetoglobulin; Human Alpha-fetoprotein; AFP

alpha-fetoprotein; Alpha-fetoprotein; alpha-fetoprotein; alpha-fetoglobulin; alpha-1-fetoprotein; alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin

AFP

AFP; AFP; FETA; HPAFP; HPAFP

FETA_HUMAN

Human

609

This assay has high sensitivity and excellent specificity for detection of Human AFP. No significant cross-reactivity or interference between Human AFP and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, cell culture supernates, tissue homogenates. Detection Range: 1.56 ng/ml-100 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 1.33 ng/ml

Sample Volume: 50-100ul. Protein Biological Process 1: Tumor marker

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to AFP. Standards or samples are then added to the appropriate microtiter plate wells with a HRP-conjugated antibody preparation specific for AFP to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain AFP, HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of AFP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

4501989

NP_001125.1

NM_001134.2

P02771

68,678 Da

Direct P53 Effectors Pathway 137939!!FOXA2 And FOXA3 Transcription Factor Networks Pathway 137911!!Glucocorticoid Receptor Regulatory Network Pathway 138014!!Hippo Signaling Pathway 749777!!Hippo Signaling Pathway 750388

This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008]

Function: Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.

96 Strip Wells

655 USD