Rat Nerve growth factor, NGF ELISA Kit | MyBioSource MBS700836 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Nerve growth factor, NGF ELISA Kit

catalog :

MBS700836

quantity :

96 Strip Wells

price :

690 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS700836

products type :

ELISA Kit

products full name :

Rat Nerve growth factor, NGF ELISA Kit

products short name :

nerve growth factor (beta polypeptide)

products name syn :

Rat Nerve growth factor; NGF ELISA kit; Beta-NGF; HSAN5; MGC161426; MGC161428; NGFB; beta-nerve growth factor; nerve growth factor; beta polypeptide; nerve growth factor; beta subunit; nerve growth factor (beta polypeptide)

other names :

beta-nerve growth factor; Beta-nerve growth factor; beta-nerve growth factor; nerve growth factor (beta polypeptide)

products gene name :

NGF

other gene names :

Ngf; Ngf; Ngfb; beta-NGF; Ngfb; Beta-NGF

uniprot entry name :

NGF_RAT

reactivity :

Rat

sequence length :

241

specificity :

This assay has high sensitivity and excellent specificity for detection of Rat NGF. No significant cross-reactivity or interference between Rat NGF and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma and tissue homogenates. Detection Range: 0.78 pg/ml-50 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 0.195 pg/ml

other info2 :

Sample Volume: 50-100ul

products description :

Introduction: Nerve growth factor (NGF), is a small secreted protein which induces the differentiation and survival of particular target neurons (nerve cells). NGF is a protein that consists of 3 types of polypeptide chains -- alpha, beta and gamma -- that interact to form the protein. The NGF beta chain (NGFB) is solely responsible for the nerve growth stimulating activity of NGF. The NGFB gene is in chromosome band 1p22. An ointment containing NGF has been reported to help heal bedsores. NGF is critical for the survival and maintenance of sympathetic and sensory neurons. NGF is released from the target cells, binds to and activates its high affinity receptor (TrkA), and is internalized into the responsive neuron. There are some data that show that NGF can be transported from the axon tip to soma, but it is unclear if this is necessary for effective cell signalling; in fact there are data showing that it is not. What is clear is that NGF binding and activation of TrkA is required for NGF-mediated neuronal survival and differentiation. NGF binds at least two receptors on the surface of cells which are capable of responding to this growth factor, TrkA and the LNGFR. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NGF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for NGF and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain NGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of NGF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

456367246

ncbi acc num :

NP_001263984.1

ncbi gb acc num :

NM_001277055.1

uniprot acc num :

P25427

ncbi mol weight :

27,010 Da

ncbi pathways :

ARMS-mediated Activation Pathway 1010106!!Activation Of TRKA Receptors Pathway 1010097!!Apoptosis Pathway 83452!!Apoptosis Pathway 470!!Axonal Growth Stimulation Pathway 1010093!!Cell Death Signalling Via NRAGE, NRIF And NADE Pathway 1010083!!Ceramide Signalling Pathway 1010091!!Frs2-mediated Activation Pathway 1010105!!Id Signaling Pathway 198536!!Inflammatory Mediator Regulation Of TRP Channels Pathway 948290

uniprot summary :

Function: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival

size :

96 Strip Wells

price :

690 USD

more info or order :

MyBioSource product webpage