ELISA/assay

Human Aspartate aminotransferase, AST ELISA Kit

MBS700831

96 Strip Wells

850 USD

ELISA Kit

Human Aspartate aminotransferase, AST ELISA Kit

glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1)

Human Aspartate aminotransferase; AST ELISA Kit; GIG18; aspartate aminotransferase 1; growth-inhibiting protein 18; glutamic-oxaloacetic transaminase 1; soluble (aspartate aminotransferase 1)

aspartate aminotransferase, cytoplasmic; Aspartate aminotransferase, cytoplasmic; aspartate aminotransferase, cytoplasmic; transaminase A; aspartate transaminase 1; aspartate aminotransferase 1; growth-inhibiting protein 18; cysteine transaminase, cytoplasmic; glutamate oxaloacetate transaminase 1; cysteine aminotransferase, cytoplasmic; glutamic-oxaloacetic transaminase 1, soluble; Cysteine aminotransferase, cytoplasmic; Cysteine transaminase, cytoplasmic; cCAT; Glutamate oxaloacetate transaminase 1; Transaminase A

GOT1

GOT1; GOT1; cCAT; GIG18; cAspAT; ASTQTL1; cAspAT; cCAT

AATC_HUMAN

Human

413

This assay has high sensitivity and excellent specificity for detection of Human GOT1. No significant cross-reactivity or interference between Human GOT1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, urine. Detection Range: 3.12 mIU/ml-200 mIU/ml. Detection Wavelength: 450 nm. Sensitivity: 0.78 mIU/ml

Sample Volume: 50-100ul

Introduction: Aspartate transaminase (AST) also called serum glutamic oxaloacetic transaminase (SGOT) or aspartate aminotransferase (ASAT/AAT) is similar to alanine transaminase (ALT) in that it is another enzyme associated with liver parenchymal cells. It is raised in acute liver damage. It is also present in red blood cells and cardiac muscle, skeletal muscle, and kidney and brain tissue, and may be elevated due to damage to those sources as well. AST was defined as a biochemical marker for the diagnosis of acute myocardial infarction in 1954. However the use of AST for such a diagnosis is now redundant and has been superseded by the cardiac troponins. AST (SGOT) is commonly measured clinically as a part of diagnostic liver function tests, to determine liver health. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to AST. Standards or samples are then added to the appropriate microtiter plate wells with a 3 biotin-conjugated antibody preparation specific for AST and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain AST, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of AST in the samples is then determined by comparing the O.D. of the samples to the standard curve.

4504067

NP_002070.1

NM_002079.2

P17174

46,248 Da

2-Oxocarboxylic Acid Metabolism Pathway 714485!!2-Oxocarboxylic Acid Metabolism Pathway 717400!!Alanine And Aspartate Metabolism Pathway 198783!!Alanine, Aspartate And Glutamate Metabolism Pathway 101142!!Alanine, Aspartate And Glutamate Metabolism Pathway 100063!!Amino Acid Synthesis And Interconversion (transamination) Pathway 106173!!Arginine And Proline Metabolism Pathway 82957!!Arginine And Proline Metabolism Pathway 323!!Biosynthesis Of Amino Acids Pathway 790012!!Biosynthesis Of Amino Acids Pathway 795174

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq, Jul 2008]

Function: Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.1 PublicationManual assertion based on experiment in:Ref.9. Catalytic activity: L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate. Cofactor: Pyridoxal phosphate.

96 Strip Wells

850 USD