catalog number :
MBS700825
products type :
ELISA Kit
products full name :
Rat Soluble Cluster of Differentiation 80, sCD80 ELISA Kit
products short name :
Soluble Cluster of Differentiation 80, sCD80
products name syn :
Rat Soluble Cluster of Differentiation 80; sCD80 ELISA Kit; Soluble Cluster of Differentiation 80; sCD80
products gene name :
sCD80
specificity :
This assay has high sensitivity and excellent specificity for detection of rat sCD80. No significant cross-reactivity or interference between rat sCD80 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 62.5 pg/ml -4000 pg/ml. Sensitivity: The minimum detectable dose of rat sCD80 is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Princple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for sCD80 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sCD80 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for sCD80 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sCD80 bound in the initial step. The color development is stopped and the intensity of the color is measured.
size4 :
10x96-Strip-Wells