catalog number :
MBS700817
products type :
ELISA Kit
products full name :
Monkey follicle-stimulating hormone, FSH ELISA Kit
products short name :
follicle-stimulating hormone, FSH
products name syn :
Monkey follicle-stimulating hormone; FSH ELISA Kit; follicle-stimulating hormone; FSH
other names :
FSH; Bromodomain-containing protein 2; O27.1.1; Really interesting new gene 3 protein
other gene names :
BRD2; KIAA9001; RING3
uniprot entry name :
BRD2_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of monkey FSH. No significant cross-reactivity or interference between monkey FSH and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Competitive. Detection Range: 0. 2 mIU/ml -80 mIU/ml. Sensitivity: The minimum detectable dose of monkey FSH is typically less than 0.2 mIU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest monkey FSH concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for FSH and Horseradish Peroxidase (HRP) conjugated FSH. The competitive inhibition reaction is launched between with HRP labeled FSH and unlabeled FSH with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of FSH in the sample. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
CAA43996.1
ncbi mol weight :
74,881 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
uniprot summary :
Function: May play a role in spermatogenesis or folliculogenesis Binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling. Regulates transcription of the CCND1 gene. Plays a role in nucleosome assembly.1 PublicationManual assertion based on experiment in:Ref.8
size4 :
10x96-Strip-Wells