ELISA/assay

Human Follistatin Like Protein 1, FSTL1 ELISA Kit

MBS700816

48-Strip-Wells

565 USD

ELISA Kit

Human Follistatin Like Protein 1, FSTL1 ELISA Kit

follistatin-like 1

Human Follistatin Like Protein 1 (FSTL1) ELISA Kit; FLJ50214; FLJ52277; FRP; FSL1; follistatin-related protein; follistatin-like 1

follistatin-related protein 1; Follistatin-related protein 1; follistatin-related protein 1; follistatin-like protein 1; follistatin-like 1; Follistatin-like protein 1

FSTL1

FSTL1; FSTL1; FRP; FSL1; MIR198; FRP

FSTL1_HUMAN

Human

308

This assay has high sensitivity and excellent specificity for detection of human FSTL1. No significant cross-reactivity or interference between human FSTL1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 12.5 ng/ml -800 ng/ml. Sensitivity: The minimum detectable dose of human FSTL1 is typically less than 3.12 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FSTL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FSTL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FSTL1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FSTL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

5901956

NP_009016.1

NM_007085.4

Q12841

34,986 Da

Signal Transduction Pathway (477114); Signaling By BMP Pathway (106336)

This gene encodes a protein with similarity to follistatin, an activin-binding protein. It contains an FS module, a follistatin-like sequence containing 10 conserved cysteine residues. This gene product is thought to be an autoantigen associated with rheumatoid arthritis. [provided by RefSeq, Jul 2008]

Function: May modulate the action of some growth factors on cell proliferation and differentiation. Binds heparin

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150