catalog number :
MBS700807
products type :
ELISA Kit
products full name :
Rat Lutenizing hormone, LH ELISA Kit
products short name :
Lutenizing hormone
products name syn :
Rat Lutenizing hormone; LH Elisa kit; Lutenizing hormone
other names :
luteinizing hormone receptor; Lutropin-choriogonadotropic hormone receptor; lutropin-choriogonadotropic hormone receptor; hypergonadotropic hypogonadism; lutropin/choriogonadotropin receptor; luteinizing hormone/choriogonadotropin receptor; Luteinizing hormone receptor; LHR; LSH-R
other gene names :
LHCGR; LHCGR; HHG; LHR; LCGR; LGR2; ULG5; LHRHR; LSH-R; LH/CGR; LH/CG-R; LCGR; LGR2; LHRHR; LH/CG-R; LHR; LSH-R
uniprot entry name :
LSHR_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of rat LH. No significant cross-reactivity or interference between rat LH and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma. Assay Type: Competitive. Detection Range: 0.3 mlU/ml -60 mlU/ml. Sensitivity: The minimum detectable dose of rat LH is typically less than 0.15 mIU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest rat LH concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for LH and Horseradish Peroxidase (HRP) conjugated LH. The competitive inhibition reaction is launched between with HRP labeled LH and unlabeled LH with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of LH in the sample. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
AAB19917.2
ncbi mol weight :
71,615 Da
ncbi pathways :
Arf6 Signaling Events Pathway (138034); Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class A Rhodopsin-like Pathway (198886); Hormone Ligand-binding Receptors Pathway (106369); Neuroactive Ligand-receptor Interaction Pathway (83053)
ncbi summary :
This gene encodes the receptor for both luteinizing hormone and choriogonadotropin. This receptor belongs to the G-protein coupled receptor 1 family, and its activity is mediated by G proteins which activate adenylate cyclase. Mutations in this gene result in disorders of male secondary sexual character development, including familial male precocious puberty, also known as testotoxicosis, hypogonadotropic hypogonadism, Leydig cell adenoma with precocious puberty, and male pseudohermaphtoditism with Leydig cell hypoplasia. [provided by RefSeq, Jul 2008]
uniprot summary :
Function: Receptor for lutropin-choriogonadotropic hormone. The activity of this receptor is mediated by G proteins which activate adenylate cyclase.
size4 :
10x96-Strip-Wells