Human tissue inhibitors of metalloproteinase 2, TIMP-2 ELISA Kit | MyBioSource MBS700796 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human tissue inhibitors of metalloproteinase 2, TIMP-2 ELISA Kit

catalog :

MBS700796

quantity :

48-Strip-Wells

price :

390 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS700796

products type :

ELISA Kit

products full name :

Human tissue inhibitors of metalloproteinase 2, TIMP-2 ELISA Kit

products short name :

TIMP metallopeptidase inhibitor 2

products name syn :

Human tissue inhibitors of metalloproteinase 2; TIMP-2 ELISA Kit; CSC-21K; tissue inhibitor of metalloproteinase 2; tissue inhibitor of metalloproteinases 2; TIMP metallopeptidase inhibitor 2

other names :

metalloproteinase inhibitor 2; Metalloproteinase inhibitor 2; metalloproteinase inhibitor 2; TIMP-2; tissue inhibitor of metalloproteinase 2; tissue inhibitor of metalloproteinases 2; TIMP metallopeptidase inhibitor 2; CSC-21K; Tissue inhibitor of metalloproteinases 2; TIMP-2

products gene name :

TIMP2

other gene names :

TIMP2; TIMP2; DDC8; CSC-21K; TIMP-2

uniprot entry name :

TIMP2_HUMAN

reactivity :

Human

sequence length :

220

specificity :

This assay has high sensitivity and excellent specificity for detection of human TIMP-2. No significant cross-reactivity or interference between human TIMP-2 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates, tissue homogenates. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: The minimum detectable dose of human TIMP-2 is typically less than 0.039 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Assay Type: Sandwich

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TIMP-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TIMP-2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TIMP-2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIMP-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4507511

ncbi acc num :

NP_003246.1

ncbi gb acc num :

NM_003255.4

uniprot acc num :

P16035

ncbi mol weight :

24,399 Da

ncbi pathways :

Activation Of Matrix Metalloproteinases Pathway (576264); Angiogenesis Pathway (198772); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262); Matrix Metalloproteinases Pathway (198900)

ncbi summary :

This gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.3 PublicationsManual assertion based on experiment in:Ref.6

size1 :

48-Strip-Wells

price1 :

390 USD

size2 :

96-Strip-Wells

price2 :

550

size3 :

5x96-Strip-Wells

price3 :

2140

size4 :

10x96-Strip-Wells

price4 :

3990

more info or order :

MyBioSource product webpage