catalog number :
MBS700794
products type :
ELISA Kit
products full name :
Pig oxytocin, OT ELISA Kit
products short name :
oxytocin, OT
products name syn :
Pig oxytocin; OT ELISA Kit; MGC126890; MGC126892; OT; OT-NPI; neurophysin I; oxytocin; prepro- (neurophysin I) ; oxytocin-neurophysin I; oxytocin-neurophysin I; preproprotein; oxytocin; OT
other names :
oxytocin; Oxytocin; Oxytocin
uniprot entry name :
E2GI53_MESAU
specificity :
This assay has high sensitivity and excellent specificity for detection of pig oxytocin. No significant cross-reactivity or interference between pig oxytocin and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Competitive. Detection Range: 3.8 pg/ml-100 pg/ml. Sensitivity: The minimum detectable dose of pig oxytocin is typically less than 3.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest pig oxytocin concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for oxytocin and Horseradish Peroxidase (HRP) conjugated oxytocin. The competitive inhibition reaction is launched between with HRP labeled oxytocin and unlabeled oxytocin with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of oxytocin in the sample. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
ADK26458.1
ncbi mol weight :
13,427 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
size4 :
10x96-Strip-Wells