ELISA/assay

Human pleiotrophin, PTN ELISA Kit

MBS700790

48-Strip-Wells

590 USD

ELISA Kit

Human pleiotrophin, PTN ELISA Kit

pleiotrophin

Human pleiotrophin; PTN ELISA Kit; HARP; HBGF8; HBNF; NEGF1; heparin affin regulatory protein; heparin binding growth factor 8; heparin-binding growth-associated molecule; neurite growth-promoting factor 1; pleiotrophin (heparin binding; pleiotrophin

pleiotrophin; Pleiotrophin; pleiotrophin; HBBM; OSF-1; HB-GAM; HBGF-8; HBNF-1; osteoblast-specific factor 1; heparin-binding brain mitogen; heparin binding growth factor 8; heparin-binding growth factor 8; heparin affin regulatory protein; neurite growth-promoting factor 1; heparin-binding growth-associated molecule; heparin-binding neurite outgrowth-promoting factor 1; pleiotrophin (heparin binding growth factor 8, neurite growth-promoting factor 1); pleiotrophin; Heparin-binding brain mitogen; HBBM; Heparin-binding growth factor 8; HBGF-8; Heparin-binding growth-associated molecule; HB-GAM; Heparin-binding neurite outgrowth-promoting factor 1; HBNF-1; Osteoblast-specific factor 1; OSF-1

PTN

PTN; PTN; HARP; HBNF; HBGF8; NEGF1; HBNF1; NEGF1; PTN; HBBM; HBGF-8; HB-GAM; HBNF-1; OSF-1

PTN_HUMAN

Human

168

This assay has high sensitivity and excellent specificity for detection of Human PTN. No significant cross-reactivity or interference between Human PTN and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates and tissue homogenates. Assay Type: Sandwich. Detection Range: 0.24 ng/ml-15 ng/ml. Sensitivity: 0.06 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for pleiotrophin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any pleiotrophin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for pleiotrophin is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of pleiotrophin bound in the initial step. The color development is stopped and the intensity of the color is measured.

4506281

NP_002816.1

NM_002825.5

P21246

18,942 Da

Syndecan-3-mediated Signaling Events Pathway (137952)

Function: Secreted growth factor that induces neurite outgrowth and which is mitogenic for fibroblasts, epithelial, and endothelial cells. Binds anaplastic lymphoma kinase (ALK) which induces MAPK pathway activation, an important step in the anti-apoptotic signaling of PTN and regulation of cell proliferation.2 PublicationsManual assertion based on experiment in:Ref.3

48-Strip-Wells

590 USD

96-Strip-Wells

850

5x96-Strip-Wells

2880

10x96-Strip-Wells

5440