catalog number :
MBS700784
products type :
ELISA Kit
products full name :
Rat Free Thyroxine, FT4 ELISA Kit
products short name :
Free Thyroxine, FT4
products name syn :
Rat Free Thyroxine; FT4 ELISA Kit; Rat Free Thyroxine; FT4
other names :
flowering locus T4, partial; Flowering locus T4; Flowering locus T4
uniprot entry name :
E3TSA0_HELAN
specificity :
This assay has high sensitivity and excellent specificity for detection of rat FT4. No significant cross-reactivity or interference between rat FT4 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma. Assay Type: Competitive. Detection Range: 4 pmol/L -64 pmol/L. Sensitivity: The minimum detectable dose of rat FT4 is typically less than 1.08 pmol/L. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest rat FT4 concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T4. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated T4. A competitive inhibition reaction is launched between T4 (Standards or samples) and Biotin-conjugated T4 with the pre-coated antibody specific for T4. The more amount of T4 in samples, the less antibody bound by Biotin-conjugated T4. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of FT4 in the sample. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
ADO61927.1
ncbi mol weight :
8,491 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
size3 :
10x96-Strip-Wells