catalog number :
MBS700773
products type :
ELISA Kit
products full name :
Chicken Interleukin 1, IL-1 ELISA Kit
products short name :
Interleukin 1, IL-1
products name syn :
Chicken Interleukin 1; IL-1 ELISA Kit; Interleukin 1; IL-1
products gene name :
IL-1
specificity :
This assay has high sensitivity and excellent specificity for detection of chicken IL-1. No significant cross-reactivity or interference between chicken IL-1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Competitive. Detection Range: 12.5 pg/ml -200 pg/ml. Sensitivity: The minimum detectable dose of chicken IL-1 is typically less than 12.5 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest chicken IL-1 concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-1. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated IL-1. A competitive inhibition reaction is launched between IL-1 (Standards or samples) and Biotin-conjugated IL-1 with the pre-coated antibody specific for IL-1. The more amount of IL-1 in samples, the less antibody bound by Biotin-conjugated IL-1. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of IL-1 in the sample. The color development is stopped and the intensity of the color is measured.
size4 :
10x96-Strip-Wells