ELISA/assay

Mouse heme oxygenase 1, HO-1 ELISA Kit

MBS700767

48-Strip-Wells

590 USD

ELISA Kit

Mouse heme oxygenase 1, HO-1 ELISA Kit

heme oxygenase (decycling) 1

Mouse heme oxygenase 1; HO-1 ELISA Kit; HO-1; HSP32; bK286B10; heme oxygenase (decyclizing) 1; heme oxygenase (decycling) 1

heme oxygenase 1; Heme oxygenase 1; heme oxygenase 1; P32 protein; hemoxygenase; heme oxygenase (decycling) 1; P32 protein

HMOX1

Hmox1; Hmox1; HO1; HO-1; Hmox; Hemox; Hsp32; D8Wsu38e; HO-1

HMOX1_MOUSE

Mouse

289

This assay has high sensitivity and excellent specificity for detection of Mouse HMOX1. No significant cross-reactivity or interference between Mouse HMOX1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates, tissue homogenates. Detection Range: 31.25 pg/ml-2000 pg/ml. Sensitivity: 7.8 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Introduction: Heme oxygenase (HO) is a microsomal enzyme that catalyzes the oxidation of heme to the antioxidant molecules, biliverdin and carbon monoxide. HO consists of two homologous isozymes, an inducible HO1 and a constitutively expressed HO2. HO1 is induced by a wide variety of stimuli including conditions of oxidative stress, inflammatory agents, transforming growth factor beta and heat shock. The increase in expression of HO1 is thought to be a cellular defense mechanism against oxidative stress since elevated HO could eventually generate more bilirubin, an anti-oxidant. HO1 expression is greatly increased in neurofibrillary tangles in Alzheimer's disease and other neurodegenerative diseases such as progressive supranuclear palsy and subacute sclerosing panencephalitis. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HO-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for HO-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain HO-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of HO-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

6754212

NP_034572.1

NM_010442.2

P14901

32,929 Da

HIF-1 Signaling Pathway (695223); Heme Degradation Pathway (1001151); Iron Uptake And Transport Pathway (1001366); Keap1-Nrf2 Pathway (198378); Metabolism Pathway (971233); Metabolism Of Porphyrins Pathway (1001149); Mineral Absorption Pathway (212238); Mineral Absorption Pathway (212220); Oxidative Stress Pathway (198384); Porphyrin And Chlorophyll Metabolism Pathway (83219)

Function: Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis. Catalytic activity: Protoheme + 3 AH2 + 3 O2 = biliverdin + Fe2+ + CO + 3 A + 3 H2O.

48-Strip-Wells

590 USD

96-Strip-Wells

850

5x96-Strip-Wells

2880

10x96-Strip-Wells

5440