ELISA/assay

Rat Steroid 5-Alpha-Reductase 1, SRD5A1 ELISA Kit

MBS700746

48-Strip-Wells

565 USD

ELISA Kit

Rat Steroid 5-Alpha-Reductase 1, SRD5A1 ELISA Kit

steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1)

Rat Steroid 5-Alpha-Reductase 1 (SRD5A1) ELISA Kit; 3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1; 5-alpha reductase; steroid 5-alpha-reductase type I; steroid-5-alpha-reductase 1; steroid-5-alpha-reductase; alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1)

3-oxo-5-alpha-steroid 4-dehydrogenase 1; 3-oxo-5-alpha-steroid 4-dehydrogenase 1; 3-oxo-5-alpha-steroid 4-dehydrogenase 1; SR type 1; steroid 5 alpha-reductase 1; steroid 5-alpha-reductase 1; steroid-5-alpha-reductase 1; steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1); SR type 1; Steroid 5-alpha-reductase 1; S5AR 1

SRD5A1

Srd5a1; Srd5a1; S5AR 1; S5AR 1

S5A1_RAT

Rat

255

This assay has high sensitivity and excellent specificity for detection of rat SRD5A1. No significant cross-reactivity or interference between rat SRD5A1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates and tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: The minimum detectable dose of rat SRD5A1 is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SRD5A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SRD5A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SRD5A1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SRD5A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

77404421

NP_058766.2

NM_017070.3

P24008

29,780 Da

Androgen Biosynthesis Pathway (1010731); Metabolism Pathway (1010675); Metabolism Of Lipids And Lipoproteins Pathway (1010682); Metabolism Of Steroid Hormones And Vitamin D Pathway (1010727); Steroid Hormone Biosynthesis Pathway (83333); Steroid Hormone Biosynthesis Pathway (301)

catalyzes the conversion of testosterone to dihydrotestosterone; required for male sex differentiation [RGD, Feb 2006]

Function: Converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology. Catalytic activity: A 3-oxo-5-alpha-steroid + NADP+ = a 3-oxo-Delta(4)-steroid + NADPH.

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150