ELISA/assay

Mouse nuclear factor- degree B p65, NF- degree B p65 ELISA Kit

MBS700743

96 Strip Wells

895 USD

ELISA Kit

Mouse nuclear factor- degree B p65, NF- degree B p65 ELISA Kit

v-rel reticuloendotheliosis viral oncogene homolog A (avian)

Mouse nuclear factor-kappaB p65; NF-kappaB p65 ELISA Kit; MGC131774; NFKB3; p65; nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene; v-rel reticuloendotheliosis viral oncogene homolog A (avian)

transcription factor p65; Transcription factor p65; transcription factor p65; p65 NFkB; p65 NF kappaB; p65 NF-kappa B; nuclear factor NF-kappa-B p65 subunit; avian reticuloendotheliosis viral (v-rel) oncogene homolog A; nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; v-rel reticuloendotheliosis viral oncogene homolog A (avian); Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3

RELA

Rela; Rela; p65; Nfkb3

TF65_MOUSE

Mouse

549

This assay has high sensitivity and excellent specificity for detection of mouse NF-kB p65. No significant cross-reactivity or interference between mouse NF-kB p65 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: For the quantitative determination of mouse nuclear factor-kappaB p65 (NF-kappaB p65) concentrations in serum, plasma, tissue homogenates. Detection Range: 31.25 pg/ml -2000 pg/ml.

Sensitivity: The minimum detectable dose of mouse NF-kB p65 is typically less than 7.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: </b<This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NF-kappaB p65 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NF-kappaB p65 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NF-kappaB p65 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NF-kappaB p65 bound in the initial step. The color development is stopped and the intensity of the color is measured.

6677709

NP_033071.1

NM_009045.4

Q04207

60,212 Da

Activated TLR4 Signalling Pathway 970703!!Activation Of NF-kappaB In B Cells Pathway 1001194!!Acute Myeloid Leukemia Pathway 83310!!Acute Myeloid Leukemia Pathway 529!!Adaptive Immune System Pathway 971210!!Adipocytokine Signaling Pathway 83290!!Adipocytokine Signaling Pathway 505!!Amoebiasis Pathway 167330!!Amoebiasis Pathway 167191!!Androgen Receptor Signaling Pathway 198319

Function: NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1.2 PublicationsManual assertion based on experiment in:Ref.14

96 Strip Wells

895 USD