Human N-acetyl-beta-D-glucosaminidase, NAG ELISA Kit | MyBioSource MBS700742 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human N-acetyl-beta-D-glucosaminidase, NAG ELISA Kit

catalog :

MBS700742

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS700742

products type :

ELISA Kit

products full name :

Human N-acetyl-beta-D-glucosaminidase, NAG ELISA Kit

products short name :

N-acetyl-beta-D-glucosaminidase, NAG

products name syn :

Human N-acetyl-beta-D-glucosaminidase; NAG ELISA Kit; ENC-1AS; N-acetyl-beta-glucosaminidase; hexosaminidase B; Beta-hexosaminidase subunit beta (HEXB) ; N-acetyl-beta-D-glucosaminidase; NAG

other names :

beta-hexosaminidase subunit beta isoform 1 preproprotein; Beta-hexosaminidase subunit beta; beta-hexosaminidase subunit beta; HCC-7; hexosaminidase subunit B; epididymis luminal protein 248; beta-N-acetylhexosaminidase subunit beta; cervical cancer proto-oncogene 7 protein; N-acetyl-beta-glucosaminidase subunit beta; hexosaminidase B (beta polypeptide); Beta-N-acetylhexosaminidase subunit beta; Hexosaminidase subunit B; Cervical cancer proto-oncogene 7 protein; HCC-7; N-acetyl-beta-glucosaminidase subunit betaCleaved into the following 2 chains:Beta-hexosaminidase subunit beta chain B; Beta-hexosaminidase subunit beta chain A

products gene name :

NAG

other gene names :

HEXB; HEXB; ENC-1AS; HEL-248; HCC7; Hexosaminidase subunit B; HCC-7

uniprot entry name :

HEXB_HUMAN

reactivity :

Human

sequence length :

556

specificity :

This assay has high sensitivity and excellent specificity for detection of human NAG. No significant cross-reactivity or interference between human NAG and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, urine, tissue homogenates. Assay Type: Competitive. Detection Range: 5 mIU/ml - 2000 mIU /ml. Sensitivity: 2 mIU/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<6%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<11%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay This assay employs the competitive inhibition enzyme immunoassay technique. Antibody specific for NAG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated NAG. A competitive inhibition reaction is launched between NAG (Standards or samples) and HRP-conjugated NAG with the pre-coated antibody specific for NAG. The more amount of NAG in samples, the less antibody bound by HRP-conjugated NAG. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of NAG in the sample. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4504373

ncbi acc num :

NP_000512.1

ncbi gb acc num :

NM_000521.3

uniprot acc num :

P07686

ncbi mol weight :

63,111 Da

ncbi pathways :

Amino Sugar And Nucleotide Sugar Metabolism Pathway (82979); Amino Sugar And Nucleotide Sugar Metabolism Pathway (350); CS/DS Degradation Pathway (645311); Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway (645308); Disease Pathway (530764); Glycogen Storage Diseases Pathway (980468); Glycosaminoglycan Degradation Pathway (82981); Glycosaminoglycan Degradation Pathway (355); Glycosaminoglycan Metabolism Pathway (645297); Glycosphingolipid Biosynthesis - Ganglio Series Pathway (82997)

ncbi summary :

Hexosaminidase B is the beta subunit of the lysosomal enzyme beta-hexosaminidase that, together with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Beta-hexosaminidase is composed of two subunits, alpha and beta, which are encoded by separate genes. Both beta-hexosaminidase alpha and beta subunits are members of family 20 of glycosyl hydrolases. Mutations in the alpha or beta subunit genes lead to an accumulation of GM2 ganglioside in neurons and neurodegenerative disorders termed the GM2 gangliosidoses. Beta subunit gene mutations lead to Sandhoff disease (GM2-gangliosidosis type II). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014]

uniprot summary :

Function: Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues. Catalytic activity: Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage