catalog number :
MBS700733
products type :
ELISA Kit
products full name :
Rat Interleukin 1 receptor antagonist, IL-1ra ELISA Kit
products short name :
interleukin 1 receptor antagonist
products name syn :
Rat Interleukin 1 receptor antagonist; IL-1ra ELISA kit; DIRA; ICIL-1RA; IL-1ra3; IL1F3; IL1RA; IRAP; MGC10430; MVCD4; IL1RN (IL1F3) ; OTTHUMP00000203730; intracellular IL-1 receptor antagonist type II; intracellular interleukin-1 receptor antagonist (icIL-1r; interleukin 1 receptor antagonist
other names :
interleukin-1 receptor antagonist protein; Interleukin-1 receptor antagonist protein; interleukin-1 receptor antagonist protein; IRAP; IL-1RN; IL-1ra; IL1 inhibitor; interleukin 1 receptor, type I; IL1 inhibitor
products gene name :
IL1RN
other gene names :
IL1RN; IL1RN; IL1R1; IL1RA; IL1RA; IL-1RN; IL-1ra; IRAP
uniprot entry name :
IL1RA_RABIT
specificity :
This assay has high sensitivity and excellent specificity for detection of Rat IL1RN. No significant cross-reactivity or interference between Rat IL1RN and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Detection Range: 125 pg/ml-8000 pg/ml. Sensitivity: 31.25 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-1RA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for IL-1RA and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain IL-1RA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IL-1RA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
NP_001076239.1
ncbi gb acc num :
NM_001082770.1
ncbi mol weight :
20,214 Da
ncbi pathways :
Cytokine Signaling In Immune System Pathway (366171); IL-1 Signaling Pathway (198908); IL1-mediated Signaling Events Pathway (137944); Immune System Pathway (106386); Interleukin-1 Signaling Pathway (160141); Signaling By Interleukins Pathway (160140)
uniprot summary :
Function: Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity.
size4 :
10x96-Strip-Wells