ELISA/assay

Mouse Endothelial nitric oxide synthase, eNOS ELISA Kit

MBS700721

96 Strip Wells

760 USD

ELISA Kit

Mouse Endothelial nitric oxide synthase, eNOS ELISA Kit

nitric oxide synthase 3 (endothelial cell)

Mouse Endothelial nitric oxide synthase; eNOS ELISA Kit; ECNOS; eNOS; nitric oxide synthase 3; nitric oxide synthase 3 (endothelial cell)

nitric oxide synthase, endothelial; Nitric oxide synthase, endothelial; nitric oxide synthase, endothelial; cNOS; EC-NOS; NOSIII; NOS type III; endothelial NOS; constitutive NOS; endothelial nitric oxide synthase; nitric oxide synthase 3, endothelial cell; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII

NOS3

Nos3; Nos3; eNOS; Nos-3; ecNOS; 2310065A03Rik; Ecnos; cNOS; eNOS; NOSIII

NOS3_MOUSE

Mouse

1202

This assay has high sensitivity and excellent specificity for detection of Mouse NOS3. No significant cross-reactivity or interference between Mouse NOS3 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates. Detection Range: 0.312 IU/ml-20 IU/ml. Detection Wavelength: 450 nm. Sensitivity: 0.078 IU/ml

Sample Volume: 50-100ul

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to eNOS. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for eNOS and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain eNOS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of eNOS in the samples is then determined by comparing the O.D. of the samples to the standard curve.

161086988

NP_032739.3

NM_008713.4

P70313

132,916 Da

ACE Inhibitor Pathway 198320!!Arginine And Proline Metabolism Pathway 83157!!Arginine And Proline Metabolism Pathway 323!!Calcium Signaling Pathway 83247!!Calcium Signaling Pathway 459!!Disease Pathway 970606!!Estrogen Signaling Pathway 799184!!Estrogen Signaling Pathway 799197!!HIF-1 Signaling Pathway 695223!!Hemostasis Pathway 1001295

Function: Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. May play a significant role in normal and abnormal limb development.1 PublicationManual assertion based on experiment in:Ref.3. Catalytic activity: 2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O. Cofactor: Heme group. Enzyme regulation: Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN

96 Strip Wells

760 USD