catalog number :
MBS700712
products type :
ELISA Kit
products full name :
Fish Insulin-like growth factors 1, IGF-1 ELISA Kit
products short name :
Insulin-like growth factors 1, IGF-1
products name syn :
Fish Insulin-like growth factors 1; IGF-1 ELISA Kit; Insulin-like growth factors 1; IGF-1
products gene name :
IGF-1
specificity :
This assay has high sensitivity and excellent specificity for detection of fish IGF-1. No significant cross-reactivity or interference between fish IGF-1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Competitive. Detection Range: 25 pg/ml-2000 pg/ml. Sensitivity: The minimum detectable dose of fish IGF-1 is typically less than 31.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest fish IGF-1 concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for IGF-1 and Horseradish Peroxidase (HRP) conjugated IGF-1. The competitive inhibition reaction is launched between with HRP labeled IGF-1 and unlabeled IGF-1 with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of IGF-1 in the sample. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
CAA01954.1
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
size4 :
10x96-Strip-Wells