ELISA/assay

Human insulin-like growth factors 1 receptor, IGF-1R ELISA Kit

MBS700695

96 Strip Wells

550 USD

ELISA Kit

Human insulin-like growth factors 1 receptor, IGF-1R ELISA Kit

insulin-like growth factor 1 receptor

Human insulin-like growth factors 1 receptor; IGF-1R ELISA Kit; CD221; IGFIR; IGFR; JTK13; MGC142170; MGC142172; MGC18216; soluble IGF1R variant 1; soluble IGF1R variant 2; insulin-like growth factor 1 receptor

insulin-like growth factor 1 receptor isoform 1; Insulin-like growth factor 1 receptor; insulin-like growth factor 1 receptor; IGF-I receptor; soluble IGF1R variant 1; soluble IGF1R variant 2; insulin-like growth factor I receptor; insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; IGF-I receptor; CD_antigen: CD221Cleaved into the following 2 chains:Insulin-like growth factor 1 receptor alpha chain; Insulin-like growth factor 1 receptor beta chain

IGF1R

IGF1R; IGF1R; IGFR; CD221; IGFIR; JTK13; IGF-I receptor

IGF1R_HUMAN

Human

1367

This assay has high sensitivity and excellent specificity for detection of human IGF-1R. No significant cross-reactivity or interference between human IGF-1R and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: For the quantitative determination of human insulin-like growth factors 1 receptor (IGF-1R) concentrations in serum, plasma, tissue homogenates. Detection Range: 0.312 ng/ml -20 ng/ml.

Sensitivity: The minimum detectable dose of human IGF-1R is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: </b<This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IGF-1R has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IGF-1R present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IGF-1R is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IGF-1R bound in the initial step. The color development is stopped and the intensity of the color is measured.

4557665

NP_000866.1

NM_000875.4

P08069

154,793 Da

AMPK Signaling Pathway 989139!!AMPK Signaling Pathway 992181!!Adherens Junction Pathway 83070!!Adherens Junction Pathway 481!!Apoptosis Pathway 198797!!Endochondral Ossification Pathway 198812!!Endocytosis Pathway 102279!!Endocytosis Pathway 102181!!Focal Adhesion Pathway 198795!!Focal Adhesion Pathway 83067

This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]

Function: Receptor tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1). Binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity. The activated IGF1R is involved in cell growth and survival control. IGF1R is crucial for tumor transformation and survival of malignant cell. Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosines phosphorylation of multiple substrates, that function as signaling adapter proteins including, the insulin-receptor substrates (IRS1/2), Shc and 14-3-3 proteins. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway and the Ras-MAPK pathway. The result of activating the MAPK pathway is increased cellular proliferation, whereas activating the PI3K pathway inhibits apoptosis and stimulates protein synthesis. Phosphorylated IRS1 can activate the 85 kDa regulatory subunit of PI3K (PIK3R1), leading to activation of several downstream substrates, including protein AKT/PKB. AKT phosphorylation, in turn, enhances protein synthesis through mTOR activation and triggers the antiapoptotic effects of IGFIR through phosphorylation and inactivation of BAD. In parallel to PI3K-driven signaling, recruitment of Grb2/SOS by phosphorylated IRS1 or Shc leads to recruitment of Ras and activation of the ras-MAPK pathway. In addition to these two main signaling pathways IGF1R signals also through the Janus kinase/signal transducer and activator of transcription pathway (JAK/STAT). Phosphorylation of JAK proteins can lead to phosphorylation/activation of signal transducers and activators of transcription (STAT) proteins. In particular activation of STAT3, may be essential for the transforming activity of IGF1R. The JAK/STAT pathway activates gene transcription and may be responsible for the transforming activity. JNK kinases can also be activated by the IGF1R. IGF1 exerts inhibiting activities on JNK activation via phosphorylation and inhibition of MAP3K5/ASK1, which is able to directly associate with the IGF1R.9 PublicationsManual assertion based on experiment in:Ref.8. Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.10 PublicationsManual assertion based on experiment in:Ref.12. Enzyme regulation: Activated by autophosphorylation at Tyr-1165, Tyr-1161 and Tyr-1166 on the kinase activation loop; phosphorylation at all three tyrosine residues is required for optimal kinase activity. Inhibited by MSC1609119A-1, BMS-754807, PQIP, benzimidazole pyridinone, isoquinolinedione, bis-azaindole, 3-cyanoquinoline, 2,4-bis-arylamino-1,3-pyrimidine, pyrrolopyrimidine, pyrrole-5-carboxaldehyde, picropodophyllin (PPP), tyrphostin derivatives. While most inhibitors bind to the ATP binding pocket, MSC1609119A-1 functions as allosteric inhibitor and binds close to the DFG motif and the activation loop.11 PublicationsManual assertion based on experiment in:Ref.29

96 Strip Wells

550 USD