Human tight junction protein 1, ZO1 ELISA Kit | MyBioSource MBS700684 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human tight junction protein 1, ZO1 ELISA Kit

catalog :

MBS700684

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS700684

products type :

ELISA Kit

products full name :

Human tight junction protein 1, ZO1 ELISA Kit

products short name :

tight junction protein 1 (zona occludens 1)

products name syn :

Human tight junction protein 1 (ZO1) ELISA Kit; DKFZp686M05161; MGC133289; ZO-1; tight junction protein 1; tight junction protein ZO-1; zona occludens 1; zonula occludens 1 protein; tight junction protein 1 (zona occludens 1)

other names :

tight junction protein ZO-1 isoform a; Tight junction protein ZO-1; tight junction protein ZO-1; zona occludens 1; zonula occludens 1 protein; tight junction protein 1; Tight junction protein 1; Zona occludens protein 1; Zonula occludens protein 1

products gene name :

TJP1

other gene names :

TJP1; TJP1; ZO-1; ZO1

uniprot entry name :

ZO1_HUMAN

reactivity :

Human

sequence length :

1748

specificity :

This assay has high sensitivity and excellent specificity for detection of human ZO1. No significant cross-reactivity or interference between human ZO1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates, Cell lysates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: The minimum detectable dose of human ZO1 is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ZO1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ZO1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZO1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZO1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

116875767

ncbi acc num :

NP_003248.3

ncbi gb acc num :

NM_003257.4

uniprot acc num :

Q07157

ncbi mol weight :

195,459 Da

ncbi pathways :

Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Apoptosis Pathway (105648); Apoptotic Cleavage Of Cell Adhesion Proteins Pathway (105680); Apoptotic Cleavage Of Cellular Proteins Pathway (105678); Apoptotic Execution Phase Pathway (105677); E-cadherin Signaling In The Nascent Adherens Junction Pathway (138005); Epithelial Cell Signaling In Helicobacter Pylori Infection Pathway (83102); Epithelial Cell Signaling In Helicobacter Pylori Infection Pathway (515); Gap Junction Pathway (83072)

ncbi summary :

This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2014]

uniprot summary :

Function: The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells.1 PublicationManual assertion based on experiment in:Ref.25

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage