ELISA/assay

Human secreted frizzled-related protein 4, SFRP4 ELISA Kit

MBS700680

48-Strip-Wells

565 USD

ELISA Kit

Human secreted frizzled-related protein 4, SFRP4 ELISA Kit

secreted frizzled-related protein 4

Human secreted frizzled-related protein 4 (SFRP4) ELISA Kit; FRP-4; FRPHE; MGC26498; secreted frizzled-related protein 4; secreted frizzled-related protein 4; secreted frizzled-related protein 4; secreted frizzled-related protein 4

secreted frizzled-related protein 4; Secreted frizzled-related protein 4; secreted frizzled-related protein 4; frizzled protein, human endometrium; secreted frizzled-related protein 4; secreted frizzled-related protein 4; secreted frizzled-related protein 4; Frizzled protein, human endometrium; FrpHE

SFRP4

SFRP4; SFRP4; FRP-4; FRPHE; sFRP-4; FRPHE; sFRP-4; FrpHE

SFRP4_HUMAN

Human

346

This assay has high sensitivity and excellent specificity for detection of Human SFRP4. No significant cross-reactivity or interference between Human SFRP4 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 1.56 ng/ml-100ng/ml. Sensitivity: 0.39 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Developmental Protein. Protein Biological Process 3: Differentiation

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SFRP4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SFRP4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SFRP4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SFRP4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

170784838

NP_003005.2

NM_003014.3

Q6FHJ7

39,827 Da

Adipogenesis Pathway (198832); Wnt Signaling Pathway (198789); Wnt Signaling Pathway (83061); Wnt Signaling Pathway (471)

Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression. [provided by RefSeq, Jul 2008]

Function: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 may act as a regulator of adult uterine morphology and function. Increases apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake.1 PublicationManual assertion based on experiment in:Ref.2

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150