ELISA/assay

Mouse thyrotropin-releasing hormone, TRH ELISA Kit

MBS700675

48-Strip-Wells

590 USD

ELISA Kit

Mouse thyrotropin-releasing hormone, TRH ELISA Kit

thyrotropin-releasing hormone

Mouse thyrotropin-releasing hormone; TRH ELISA KIT; MGC125964; MGC125965; ; thyrotropin-releasing hormone

thyrotropin releasing hormone preproprotein; Pro-thyrotropin-releasing hormone; thyrotropin releasing hormone; protirelin; prothyroliberin; TSH-releasing factor; thyrotropin-releasing factor; pro-thyrotropin-releasing hormone; thyrotropin releasing hormone; ProthyroliberinCleaved into the following chain:Thyrotropin-releasing hormone; TRHAlternative name(s):Protirelin; TSH-releasing factor; Thyroliberin; Thyrotropin-releasing factor; TRF

TRH

Trh; Trh; Trf; Pro-TRH; Pro-TRH; TRH; TRF

TRH_MOUSE

Mouse

256

This assay has high sensitivity and excellent specificity for detection of Mouse TRH. No significant cross-reactivity or interference between Mouse TRH and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum. Assay Type: Sandwich. Detection Range: 2.5 pg/ml-160 pg/ml. Sensitivity: 0.625 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

For Sample For the quantitative determination of mouse thyrotropin-releasing hormone (TRH) concentrations in serum. Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TRH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TRH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRH is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRH bound in the initial step. The color development is stopped and the intensity of the color is measured.

134031937

NP_033452.2

NM_009426.3

Q62361

29,195 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (1000942); G Alpha (q) Signalling Events Pathway (1000975); GPCR Downstream Signaling Pathway (1000971); GPCR Ligand Binding Pathway (1000941); Gastrin-CREB Signalling Pathway Via PKC And MAPK (970815); Peptide Ligand-binding Receptors Pathway (1000943); Signal Transduction Pathway (970540); Signaling By GPCR Pathway (971377)

This gene encodes a member of the thyrotropin-releasing hormone family. Cleavage of the encoded proprotein releases mature thyrotropin-releasing hormone, which is a tripeptide hypothalamic regulatory hormone. The mouse proprotein contains five thyrotropin-releasing hormone tripeptides. Thyrotropin-releasing hormone is involved in the regulation and release of thyroid-stimulating hormone, as well as prolactin. Disruption of this gene results in hypothyroidism, elevated thyroid-stimulating hormone levels, and hyperglycemia. [provided by RefSeq, Apr 2013]

Function: Functions as a regulator of the biosynthesis of TSH in the anterior pituitary gland and as a neurotransmitter/ neuromodulator in the central and peripheral nervous systems.

48-Strip-Wells

590 USD

96-Strip-Wells

850

5x96-Strip-Wells

2880

10x96-Strip-Wells

5440