Human diamine oxidase, DAO ELISA Kit | MyBioSource MBS700670 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human diamine oxidase, DAO ELISA Kit

catalog :

MBS700670

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS700670

products type :

ELISA Kit

products full name :

Human diamine oxidase, DAO ELISA Kit

products short name :

D-amino-acid oxidase

products name syn :

Human diamine oxidase; DAO ELISA Kit; DAAO; DAMOX; MGC35381; OXDA; ; D-amino-acid oxidase

other names :

D-amino-acid oxidase; D-amino-acid oxidase; D-amino-acid oxidase; D-amino acid oxidase; D-amino-acid oxidase

products gene name :

DAO

other gene names :

DAO; DAO; DAAO; OXDA; DAMOX; DAMOX; DAAO; DAMOX; DAO

uniprot entry name :

OXDA_HUMAN

reactivity :

Human

sequence length :

347

specificity :

This assay has high sensitivity and excellent specificity for detection of Human DAO. No significant cross-reactivity or interference between Human DAO and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Detection Range: 15.6 mIU/ml-1000 mIU/ml. Sensitivity: 3.9 mIU/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

products description :

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with anantibody specific to DAO. Standards or samples are then added to theappropriate microtiter plate wells with a biotin-conjugated polyclonalantibody preparation specific for DAO and Avidin conjugated to HorseradishPeroxidase (HRP) is added to each microplate well and incubated. Then aTMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to eachwell. Only those wells that contain DAO, biotin-conjugated antibody andenzyme-conjugated Avidin will exhibit a change in color. Theenzyme-substrate reaction is terminated by the addition of a sulphuric acidsolution and the color change is measured spectrophotometrically at awavelength of 450 nm +/- 2 nm. The concentration of DAO in the samples isthen determined by comparing the O.D. of the samples to the standardcurve.

ncbi gi num :

148539837

ncbi acc num :

NP_001908.3

ncbi gb acc num :

NM_001917.4

uniprot acc num :

P14920

ncbi mol weight :

39,474 Da

ncbi pathways :

Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); D-Arginine And D-ornithine Metabolism Pathway (82972); D-Arginine And D-ornithine Metabolism Pathway (341); Glycine, Serine And Threonine Metabolism Pathway (82949); Glycine, Serine And Threonine Metabolism Pathway (313); Glyoxylate Metabolism Pathway (160990); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Amino Acids And Derivatives Pathway (106169)

ncbi summary :

This gene encodes the peroxisomal enzyme D-amino acid oxidase. The enzyme is a flavoprotein which uses flavin adenine dinucleotide (FAD) as its prosthetic group. Its substrates include a wide variety of D-amino acids, but it is inactive on the naturally occurring L-amino acids. Its biological function is not known; it may act as a detoxifying agent which removes D-amino acids that accumulate during aging. In mice, it degrades D-serine, a co-agonist of the NMDA receptor. This gene may play a role in the pathophysiology of schizophrenia. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Regulates the level of the neuromodulator D-serine in the brain. Has high activity towards D-DOPA and contributes to dopamine synthesis. Could act as a detoxifying agent which removes D-amino acids accumulated during aging. Acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. Does not act on acidic amino acids.1 PublicationManual assertion based on experiment in:Ref.8. Catalytic activity: A D-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2.1 PublicationManual assertion based on experiment in:Ref.7. Cofactor: FAD.

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage