catalog number :
MBS700661
products type :
ELISA Kit
products full name :
Human osteocrin, Ostn ELISA Kit
products short name :
osteocrin
products name syn :
Human osteocrin; Ostn ELISA kit; MUSCLIN; ; osteocrin
other names :
osteocrin; Osteocrin; osteocrin; osteocrin; Musclin
products gene name :
OSTN
other gene names :
OSTN; OSTN; MUSCLIN
uniprot entry name :
OSTN_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of Human OSTN. No significant cross-reactivity or interference between Human OSTN and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma and tissue homogenates. Detection Range: 0.312 ng/ml-20 ng/ml. Sensitivity: 0.078 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Developmental Protein. Protein Biological Process 3: Differentiation
products description :
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ostn. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Ostn and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain Ostn, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Ostn in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
NP_937827.1
ncbi gb acc num :
NM_198184.1
ncbi mol weight :
14,722 Da
uniprot summary :
Function: Appears to modulate osteoblastic differentiation. Could also function as an autocrine and paracrine factor linked to glucose metabolism in skeletal muscle
size4 :
10x96-Strip-Wells