ELISA/assay

Human cytovillin/ezrin ELISA Kit

MBS700648

96 Strip Wells

760 USD

ELISA Kit

Human cytovillin/ezrin ELISA Kit

ezrin

Human cytovillin/ezrin ELISA Kit; CVIL; CVL; DKFZp762H157; FLJ26216; MGC1584; VIL2; cytovillin 2; villin 2 (ezrin) ; ezrin

ezrin; Ezrin; ezrin; p81; villin-2; cytovillin 2; villin 2 (ezrin); epididymis secretory protein Li 105; ezrin; Cytovillin; Villin-2; p81

EZR

EZR; EZR; CVL; CVIL; VIL2; HEL-S-105; VIL2

EZRI_HUMAN

Human

586

This assay has high sensitivity and excellent specificity for detection of Human EZR. No significant cross-reactivity or interference between Human EZR and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates and cell lysates. Detection Range: 0.312 ng/ml-20 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.078 ng/ml

Sample Volume: 50-100ul. Protein Biological Process 1: Cell Cycle. Protein Biological Process 3: Cell shape

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for cytovillin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any cytovillin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for cytovillin is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of cytovillin bound in the initial step. The color development is stopped and the intensity of the color is measured.

161702986

NP_001104547.1

NM_001111077.1

P15311

69,413 Da

AGE/RAGE Pathway 698754!!Axon Guidance Pathway 105688!!Developmental Biology Pathway 477129!!FAS (CD95) Signaling Pathway 138081!!Gastric Acid Secretion Pathway 154409!!Gastric Acid Secretion Pathway 154383!!L1CAM Interactions Pathway 161003!!LKB1 Signaling Events Pathway 137957!!Leukocyte Transendothelial Migration Pathway 83083!!Leukocyte Transendothelial Migration Pathway 494

The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. [provided by RefSeq, Jul 2008]

Function: Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.3 PublicationsManual assertion based on experiment in:Ref.17. Enzyme regulation: A head-to-tail association, of the N-terminal and C-terminal halves results in a closed conformation (inactive form) which is incapable of actin or membrane-binding

96 Strip Wells

760 USD