catalog number :
MBS700631
products type :
ELISA Kit
products full name :
Mouse Transforming Growth factor beta3, TGF-beta3 ELISA Kit
products short name :
transforming growth factor, beta 3
products name syn :
Mouse Transforming Growth factor beta3; TGF-beta3 ELISA kit; ARVD; FLJ16571; TGF-beta3; ; transforming growth factor; beta 3
other names :
Transforming growth factor beta-3; Transforming growth factor beta-3; transforming growth factor beta-3; TGF-beta-3; transforming growth factor, beta 3
products gene name :
TGFB3
other gene names :
Tgfb3; Tgfb3; Tgfb-3; TGF-beta-3; LAP
uniprot entry name :
TGFB3_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse TGF-beta3. No significant cross-reactivity or interference between mouse TGF-beta3 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates and tissue homogenates. Assay Type: Sandwich. Detection Range: 125 pg/ml -8000 pg/ml. Sensitivity: 31.25 pg/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TGF-beta3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TGF-beta3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TGF-beta3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TGF-beta3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi mol weight :
46,885 Da
ncbi pathways :
Amoebiasis Pathway (167330); Amoebiasis Pathway (167191); Cell Cycle Pathway (83251); Cell Cycle Pathway (463); Chagas Disease (American Trypanosomiasis) Pathway (147810); Chagas Disease (American Trypanosomiasis) Pathway (147795); Chronic Myeloid Leukemia Pathway (83309); Chronic Myeloid Leukemia Pathway (528); Colorectal Cancer Pathway (83299); Colorectal Cancer Pathway (518)
ncbi summary :
This gene encodes a member of the transforming growth factor (TGF) beta superfamily of cell regulatory proteins that control adhesion, proliferation, differentiation and transformation of many cell types. The encoded protein likely undergoes proteolytic cleavage similar to the human ortholog to generate an active peptide from the C-terminal region (TGF-beta 3), which is rendered inactive by its non-covalent association with the N-terminal peptide called the "Latency-associated peptide." Mice lacking the encoded protein die shortly after birth, and display abnormal pulmonary development and defective palatogenesis. [provided by RefSeq, Jul 2015]
uniprot summary :
Function: Involved in embryogenesis and cell differentiation.
size4 :
10x96-Strip-Wells