catalog number :
MBS700630
products type :
ELISA Kit
products full name :
Rat Macrophage Colony-Stimulating Factor, M-CSF ELISA Kit
products short name :
colony stimulating factor 1 (macrophage)
products name syn :
Rat Macrophage Colony-Stimulating Factor; M-CSF ELISA kit; MCSF; MGC31930; OTTHUMP00000013363; OTTHUMP00000013364; colony stimulating factor 1; macrophage colony stimulating factor; colony stimulating factor 1 (macrophage)
other names :
Macrophage colony-stimulating factor 1; Macrophage colony-stimulating factor 1; macrophage colony-stimulating factor 1; MCSF; CSF-1; colony stimulating factor 1 (macrophage)
products gene name :
CSF1
other gene names :
Csf1; Csf1; Csfm; CSF-1; MCSF
uniprot entry name :
CSF1_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of Rat CSF1. No significant cross-reactivity or interference between Rat CSF1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: 3.9 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
For Sample For the quantitative determination of rat macrophage colony-stimulating factor (M-CSF) concentrations in serum, plasma, cell culture supernates, tissue homogenates. PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for M-CSF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any M-CSF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for M-CSF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of M-CSF bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi mol weight :
62,187 Da
ncbi pathways :
Cytokine-cytokine Receptor Interaction Pathway (83443); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response (BioCarta) Pathway (198521); Hematopoietic Cell Lineage Pathway (83470); Hematopoietic Cell Lineage Pathway (489); Osteoclast Differentiation Pathway (193140); Osteoclast Differentiation Pathway (193096); PI3K-Akt Signaling Pathway (692247); PI3K-Akt Signaling Pathway (692979); Rap1 Signaling Pathway (869320)
ncbi summary :
plays a role in macrophage formation; involved in osteoclastogenesis and endochondral ossification [RGD, Feb 2006]
uniprot summary :
Function: Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance
size4 :
10x96-Strip-Wells