catalog number :
MBS700622
products type :
ELISA Kit
products full name :
Rat Alpha-fetoprotein, AFP ELISA Kit
products short name :
alpha-fetoprotein
products name syn :
Rat Alpha-fetoprotein; AFP ELISA Kit; FETA; HPAFP; OTTHUMP00000160480; alpha-1-fetoprotein; alpha-fetoglobulin; alpha-fetoprotein
other names :
Alpha-fetoprotein; Alpha-fetoprotein; alpha-fetoprotein; alpha-fetoglobulin; alpha-1-fetoprotein; chloride intracellular channel 6; alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin
other gene names :
Afp; Afp
uniprot entry name :
FETA_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of Rat AFP. No significant cross-reactivity or interference between Rat AFP and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: 3.9 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AFP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AFP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AFP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AFP bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi mol weight :
68,386 Da
ncbi pathways :
Hippo Signaling Pathway (749790); Hippo Signaling Pathway (750388)
ncbi summary :
plasma protein expressed in the fetal liver and yolk sac [RGD, Feb 2006]
uniprot summary :
Function: Binds estrogens, fatty acids and metals.
size4 :
10x96-Strip-Wells