ELISA/assay

Bovine Interleukin 2, IL-2 ELISA Kit

MBS700611

24-Strip-Wells (LIMIT 1)

240 USD

ELISA Kit

Bovine Interleukin 2, IL-2 ELISA Kit

[interleukin 2]

[Bovine Interleukin 2; IL-2 ELISA Kit; IL-2; TCGF; lymphokine; T cell growth factor; aldesleukin; interleukin-2; involved in regulation of T-cell clonal expansion; interleukin 2]

[interleukin-2; Interleukin-2; interleukin-2; T-cell growth factor; interleukin 2<; T-cell growth factor; TCGF]

[IL2]

[IL2; IL2; IL-2; TCGF; IL-2; IL-2; TCGF]

IL2_BOVIN

Bovine

155

This assay has high sensitivity and excellent specificity for detection of Bovine IL2. No significant cross-reactivity or interference between Bovine IL2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Detection Range: 31.25 pg/ml-2000 pg/ml. Sensitivity: 7.8 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Immunity. Protein Biological Process 3: Adaptive immunity

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for IL-2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain IL-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IL-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

379991150

NP_851340.2

NM_180997.2

P05016

17,628 Da

Allograft Rejection Pathway (84358); Allograft Rejection Pathway (535); Autoimmune Thyroid Disease Pathway (84356); Autoimmune Thyroid Disease Pathway (533); Chagas Disease (American Trypanosomiasis) Pathway (147808); Chagas Disease (American Trypanosomiasis) Pathway (147795); Cytokine Signaling In Immune System Pathway (1009189); Cytokine-cytokine Receptor Interaction Pathway (84290); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response (BioCarta) Pathway (198574)

Function: Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.

24-Strip-Wells (LIMIT 1)

240 USD

48-Strip-Wells

420

96-Strip-Wells

600

5x96-Strip-Wells

2495

10x96-Strip-Wells

4715