ELISA/assay

Human Brain derived neurotrophic factor, BDNF ELISA Kit

MBS700602

96-Strip-Wells

630 USD

ELISA Kit

Human Brain derived neurotrophic factor, BDNF ELISA Kit

brain-derived neurotrophic factor

Human Brain derived neurotrophic factor; BDNF ELISA Kit; MGC34632; neurotrophin; Human Brain derived neurotrophic factor; BDNF

brain-derived neurotrophic factor isoform a preproprotein; Brain-derived neurotrophic factor; brain-derived neurotrophic factor; abrineurin; neurotrophin; brain-derived neurotrophic factor; Abrineurin

BDNF

BDNF; BDNF; ANON2; BULN2; BDNF

BDNF_HUMAN

Human

247

This assay has high sensitivity and excellent specificity for detection of human BDNF. No significant cross-reactivity or interference between human BDNF and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Quantitative Sandwich. Sensitivity: Typically less than 0.063 ng/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BDNF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BDNF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BDNF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BDNF bound in the initial step. The color development is stopped and the intensity of the color is measured.

219842288

NP_001137277.1

NM_001143805.1

P23560

27,818 Da

Alcoholism Pathway (585563); Alcoholism Pathway (587116); BDNF Signaling Pathway (712093); Cocaine Addiction Pathway (546258); Cocaine Addiction Pathway (546273); FSH Signaling Pathway (672455); Huntington's Disease Pathway (83100); Huntington's Disease Pathway (512); Integrated Pancreatic Cancer Pathway (711360); MAPK Signaling Pathway (198779)

The protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Jan 2009]

Function: During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.1 PublicationManual assertion based on experiment in:Ref.24

96-Strip-Wells

630 USD

5x96-Strip-Wells

1955

10x96-Strip-Wells

3635